Hydrogen suppresses the progression of lung cancerScientific Research

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original title: Hydrogen gas represses the progression of lung cancer via down-regulating CD47


Jinghong Meng; Leyuan Liu; Dongchang Wang; Zhenfeng Yan; Gang Chen

DOI: 10.1042/BSR20192761

Published on: 28/04/2020


Hydrogen (H2) has been found to exert antitumor effects in several types of cancer, but its molecular mechanisms remain largely unknown. In our previous study, our project group found that H2 may reduce the expression of CD47 in lung cancer A549 cells through next-generation sequencing, suggesting that CD47 may be involved in H2-mediated lung cancer suppression. Therefore, this study aimed to investigate the effect of CD47 on H2-induced lung cancer suppression. Western blotting and real-time PCR (RT-PCR) assays were used to detect protein and mRNA levels, respectively. Cell proliferation, invasion, migration and apoptosis were detected using Cell Counting Kit-8 (CCK-8), Transwell chamber, wound healing and flow cytometry, respectively. The results showed that H2 treatment resulted in a dose-dependent decrease in the expression levels of CD47 and cell division control protein 42 (CDC42).

Upregulation of CD47 abolished the role of H2 in promoting lung cancer cell apoptosis and inhibiting cell growth, invasion and migration in A549 and H1975 cell lines. However, CD47 knockdown enhanced the role of H2 in suppressing lung cancer. In addition, we also observed that H2 treatment induced marked suppression of CDC42 and CD47 expression levels in mouse tumor tissues and enhanced macrophage-mediated phagocytosis in A549 and H1975 cells. In conclusion, the current study shows that H2 inhibits lung cancer progression through CD47 downregulation, which may be an effective approach for the treatment of lung cancer.

Research Conclusion

In conclusion, the present study makes clear that H2 exerts an anti-tumor role in lung cancer via down-regulating CD47, an antiphagocytic molecule. As more and more attention has been attracted on the application of H2 therapy in clinical trials [32–34], H2 therapy should be proposed as a promising therapeutic strategy for cancer treatment.

Original Publication