The effect of hydrogen gas on the behavior of gastric cancer cellsScientific Research
Hydrogen inhibits the proliferation and migration of gastric cancer cells by modulating lncRNA MALAT1/miR-124-3p/EZH2 axis
Gastric cancer is one of the most common and deadly malignancies with no effective treatment options. This study aimed to investigate the effect of hydrogen on the behavior of gastric cancer cells.
Gastric cancer cell lines MGC-803 and BGC-823 were treated with or without H2/O2 gas mixture (66.7%:33.3% v/v). Proliferation and migration were assessed by MTT and scratch wound healing assays, respectively. The expression of lncRNA MALAT1, miR-124-3p and EZH2 was analyzed by real-time quantitative PCR and/or Western blotting. Tumor growth was estimated using a xenograft mouse model.
H2 gas significantly inhibited gastric tumor growth and gastric cancer cell proliferation, migration and lncRNA MALAT1 and EZH2 expression in vivo, while miR-124-3p expression was upregulated. Overexpression of LncRNA MALAT1 abolished all of the aforementioned effects of H2. LncRNA MALAT1 and miR-124-3p mutually inhibited the expression. MiR-124-3p mimics the disabled lncRNA MALAT1 and promotes EZH2 expression and gastric cancer cell proliferation and migration.
These data suggest that H2 can be used as a therapeutic agent in gastric cancer and the lncRNA MALAT1/miR-124-3p/EZH2 axis can be a target for intervention.
Cancer Cell International volume 21, Article number: 70 (2021)