{"id":26756,"date":"2024-01-03T21:38:06","date_gmt":"2024-01-03T19:38:06","guid":{"rendered":"https:\/\/hho-bulgaria.com\/hydrogen-inhalation-reduces-lung-injury-in-rats\/"},"modified":"2024-01-29T21:13:21","modified_gmt":"2024-01-29T19:13:21","slug":"hydrogen-inhalation-reduces-lung-injury-in-rats","status":"publish","type":"post","link":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/","title":{"rendered":"Hydrogen Inhalation Reduces Lung Injury in Rats"},"content":{"rendered":"<div class=\"jig-ncbiinpagenav\" data-jigconfig=\"smoothScroll: false, allHeadingLevels: ['h2'], headingExclude: ':hidden,.nomenu'\" id=\"ui-ncbiinpagenav-1\">\n<div class=\"fm-sec half_rhythm no_top_margin\">\n<div class=\"fm-flexbox\">\n<div class=\"fm-citation\">\n<div class=\"citation-default\">\n<div class=\"part1\"><span role=\"menubar\"><a href=\"#\" role=\"menuitem\" aria-expanded=\"false\" aria-haspopup=\"true\">Front Physiol.<\/a><\/span> 2021; 12: 699344. <\/div>\n<div class=\"part2\"><span class=\"fm-vol-iss-date\">Published online 2021 Aug 2. <\/span>  <span class=\"doi\"><span>doi:&nbsp;<\/span><a href=\"\/\/doi.org\/10.3389%2Ffphys.2021.699344\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CFront%20Matter&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">10.3389\/fphys.2021.699344<\/a><\/span><\/div>\n<\/div>\n<\/div>\n<div class=\"fm-ids\">\n<div class=\"fm-citation-pmcid\"><span class=\"fm-citation-ids-label\">PMCID: <\/span><span>PMC8365359<\/span><\/div>\n<div class=\"fm-citation-pmid\">PMID: <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34408660\">34408660<\/a><\/div>\n<\/div>\n<\/div>\n<h1 class=\"content-title\">Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats<\/h1>\n<div class=\"half_rhythm\">\n<div class=\"contrib-group fm-author\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zheng%20P%5BAuthor%5D\">Panpan Zheng<\/a>,<sup><br \/>\n\u2020<br \/>\n<\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Kang%20J%5BAuthor%5D\">Jiyu Kang<\/a>,<sup><br \/>\n\u2020<br \/>\n<\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Xing%20E%5BAuthor%5D\">Entong Xing<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zheng%20B%5BAuthor%5D\">Bin Zheng<\/a>, <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Wang%20X%5BAuthor%5D\">Xueyao Wang<\/a>, and  <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=Zhou%20H%5BAuthor%5D\">Huacheng Zhou<\/a><sup><br \/>\n*<br \/>\n<\/sup><\/div>\n<div style=\"display:none\" class=\"contrib-group aff-tip\"><\/div>\n<\/div>\n<div class=\"half_rhythm\">\n<div class=\"togglers fm-copyright-license\"><a href=\"#\" class=\"pmctoggle\" rid=\"idm140623238698368_ai\">Author information<\/a> <a href=\"#\" class=\"pmctoggle\" rid=\"idm140623238698368_an\">Article notes<\/a> <a href=\"#\" class=\"pmctoggle\" rid=\"idm140623238698368_cpl\">Copyright and License information<\/a> <a href=\"\/pmc\/about\/disclaimer\/\" style=\"margin-left: 1em\">PMC Disclaimer<\/a><\/div>\n<div class=\"fm-authors-info hide half_rhythm\" id=\"idm140623238698368_ai\" style=\"display:none\">\n<div class=\"fm-affl\" id=\"aff-a.m.b.e\">Department of Anesthesiology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, China<\/div>\n<div id=\"fn-a.m.b.f.a\">Edited by: Walter Araujo Zin, Federal University of Rio de Janeiro, Brazil<\/div>\n<div id=\"fn-a.m.b.f.b\">Reviewed by: Sam Bayat, Universit\u00e9 Grenoble Alpes, France; Jill Johnson, Aston University, United Kingdom<\/div>\n<div id=\"c001\">*Correspondence: Huacheng Zhou, <a href=\"mailto:dev@null\" data-email=\"moc.361@gnehcauhuohz\" class=\"oemail\">moc.361@gnehcauhuohz<\/a><\/div>\n<div id=\"fn002\"><sup>\u2020<\/sup>These authors have contributed equally to this work and share first authorship<\/div>\n<div id=\"fn004\">This article was submitted to Respiratory Physiology, a section of the journal Frontiers in Physiology<\/div>\n<\/div>\n<div class=\"fm-article-notes hide half_rhythm\" id=\"idm140623238698368_an\" style=\"display:none\">\n<div class=\"fm-pubdate half_rhythm\">Received 2021 Apr 23; Accepted 2021 Jul 8.<\/div>\n<\/div>\n<div class=\"permissions half_rhythm hide\" id=\"idm140623238698368_cpl\" style=\"display:none\">\n<div class=\"fm-copyright half_rhythm\"><a href=\"\/pmc\/about\/copyright\/\">Copyright<\/a>  \u00a9 2021 Zheng, Kang, Xing, Zheng, Wang and Zhou.<\/div>\n<div class=\"license half_rhythm\">This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.<\/div>\n<\/div>\n<\/div>\n<div id=\"pmclinksbox\" class=\"links-box whole_rhythm hidden\" role=\"complementary\" aria-label=\"Related or updated information about this article.\"><\/div>\n<\/div>\n<div class=\"sec\"><\/div>\n<div id=\"ass-data\" class=\"tsec fm-sec whole_rhythm\" data-section=\"Featured_PMC_Datacitation\">\n<h2 class=\"nomenu\">Associated Data<\/h2>\n<dl data-count=\"2\" class=\"box-data-suppmats whole_rhythm no_bottom_margin\">\n<dt><a href=\"#\" rid=\"data-suppmats\" data-ga-action=\"click_feat_toggler\" data-ga-label=\"Supplementary Materials\" class=\"pmctoggle\">Supplementary Materials<\/a><\/dt>\n<dd id=\"data-suppmats\" style=\"display: none;\">\n<div class=\"half_rhythm\">\n<div><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/bin\/Table_1.DOC\" data-ga-action=\"click_feat_suppl\">Table_1.DOC<\/a><span style=\"color:gray\"> (45K)<\/span><\/div>\n<div class=\"small guid\">GUID:&nbsp;4D25681C-E135-4B2C-8F95-17B0F21064F8<\/div>\n<\/div>\n<div class=\"half_rhythm\">\n<div><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/bin\/Table_2.DOC\" data-ga-action=\"click_feat_suppl\">Table_2.DOC<\/a><span style=\"color:gray\"> (32K)<\/span><\/div>\n<div class=\"small guid\">GUID:&nbsp;3E180932-98CA-4BC4-AEB6-B1264DBED79B<\/div>\n<\/div>\n<\/dd>\n<\/dl>\n<dl data-length=\"166\" class=\"box-data-avail whole_rhythm no_bottom_margin\">\n<dt><a href=\"#\" rid=\"data-avl-stmnt\" data-ga-action=\"click_feat_toggler\" data-ga-label=\"Data Availability Statement\" class=\"pmctoggle\">Data Availability Statement<\/a><\/dt>\n<dd id=\"data-avl-stmnt\" style=\"display: none;\">\n<p class=\"p p-first-last\">The original contributions presented in the study are included in the article\/<a href=\"#TS1\" rid=\"TS1\" class=\" supplementary-material\">Supplementary Material<\/a>, further inquiries can be directed to the corresponding author\/s.<\/p>\n<\/dd>\n<\/dl>\n<\/div>\n<div id=\"abstract-a.m.b.m\" lang=\"en\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><span role=\"menubar\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"menuitem\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/span><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"abstract-a.m.b.mtitle\">Abstract<\/h2>\n<p><!--article-meta--><\/p>\n<div>\n<p class=\"p p-first\"><strong>Background:<\/strong> Lung inflation with hydrogen is an effective method to protect donor lungs from lung ischemia-reperfusion injury (IRI). This study aimed to examine the effect of lung inflation with 3% hydrogen during the cold ischemia phase on pyroptosis in lung grafts of rats.<\/p>\n<p><strong>Methods:<\/strong> Adult male Wistar rats were randomly divided into the sham group, the control group, the oxygen (O<sub>2<\/sub>) group, and the hydrogen (H<sub>2<\/sub>) group. The sham group underwent thoracotomy but no lung transplantation. In the control group, the donor lungs were deflated for 2 h. In the O<sub>2<\/sub> and H<sub>2<\/sub> groups, the donor lungs were inflated with 40% O<sub>2<\/sub> + 60% N<sub>2<\/sub> and 3% H<sub>2<\/sub> + 40% O<sub>2<\/sub> + 57% N<sub>2<\/sub>, respectively, at 10 ml\/kg, and the gas was replaced every 20 min during the cold ischemia phase for 2 h. Two hours after orthotopic lung transplantation, the recipients were euthanized.<\/p>\n<p><strong>Results:<\/strong> Compared with the control group, the O<sub>2<\/sub> and H<sub>2<\/sub> groups improved oxygenation indices, decreases the inflammatory response and oxidative stress, reduced lung injury, and improved pressure-volume (P-V) curves. H<sub>2<\/sub> had a better protective effect than O<sub>2<\/sub>. Furthermore, the levels of the pyroptosis-related proteins selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase (caspase)-1 p20, and the N-terminal of gasdermin D (GSDMD-N) were decreased in the H<sub>2<\/sub> group.<\/p>\n<p class=\"p p-last\"><strong>Conclusion:<\/strong> Lung inflation with 3% hydrogen during the cold ischemia phase inhibited the inflammatory response, oxidative stress, and pyroptosis and improved the function of the graft. Inhibiting reactive oxygen species (ROS) production may be the main mechanism of the antipyroptotic effect of hydrogen.<\/p>\n<\/div>\n<div class=\"sec\"><strong class=\"kwd-title\">Keywords: <\/strong><span class=\"kwd-text\">lung transplantation, hydrogen, pyroptosis, lung inflation, cold ischemia phase<\/span><\/div>\n<\/div>\n<div id=\"S1\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S1title\">Introduction<\/h2>\n<p class=\"p p-first\">The preservation of donor lungs during the cold ischemia phase (CIP) is crucial for prognosis after lung transplantation (LTx). The conventional preservation methods for donor lungs include lung preservation solution and perfusion solution, hypothermic preservation (4\u20138\u00b0C), and lung inflation with oxygen or air (<a href=\"#B2\" rid=\"B2\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">de Perrot and Keshavjee, 2004<\/a>). However, these methods are not sufficient for lung preservation. Recently, <em>ex vivo<\/em> lung perfusion (EVLP), as a modern technique for preserving donor lungs, was shown to be better for preserving lung function, but this method requires complicated and special equipment (<a href=\"#B13\" rid=\"B13\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Machuca et al., 2013<\/a>).<\/p>\n<p>The application of hydrogen for organ transplantation has been extensively studied, and hydrogen can alleviate graft injury during heart, liver, kidney, and lung transplantation (<a href=\"#B18\" rid=\"B18\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Noda et al., 2013<\/a&gt;; <a href=\"#B10\" rid=\"B10\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Kobayashi and Sano, 2019<\/a&gt;; <a href=\"#B29\" rid=\"B29\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Uto et al., 2019<\/a&gt;; <a href=\"#B26\" rid=\"B26\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Saito et al., 2020<\/a>). Ventilation of donor lungs with 2% hydrogen during EVLP can improve the quality of the lung graft in rats (<a href=\"#B17\" rid=\"B17\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Noda et al., 2014<\/a>). The protective effect of hydrogen on donor lungs could expand the donor pool to brain death donors and cardiac death donors and alleviate the shortage of donor lungs in rats (<a href=\"#B35\" rid=\"B35\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Zhou et al., 2013<\/a&gt;; <a href=\"#B34\" rid=\"B34\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Zhang et al., 2019<\/a>). In mouse allogeneic tracheal transplantation, oral hydrogen-saturated water daily suppresses the development of mid-term obliterative airway disease (<a href=\"#B20\" rid=\"B20\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Ozeki et al., 2020<\/a>). Anti-inflammatory, antioxidative, and antiapoptotic effects are mainly mechanisms that hydrogen alleviates lung injury (<a href=\"#B9\" rid=\"B9\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Kawamura et al., 2010<\/a>).<\/p>\n<p class=\"p p-last\">Pyroptosis plays an important role in donor lung injury, and inhibiting pyroptosis could improve the quality of donor lungs (<a href=\"#B19\" rid=\"B19\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Noda et al., 2017<\/a>). Lung ischemia-reperfusion injury (IRI) could be alleviated by inhibiting the selective nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome, which is involved in the classical pyroptosis pathway (<a href=\"#B31\" rid=\"B31\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Xu et al., 2018<\/a>). Hydrogen-rich water can inhibit pyroptosis and improve acute pancreatitis by inhibiting NLRP3 inflammasome activation (<a href=\"#B24\" rid=\"B24\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Ren et al., 2014<\/a>). However, the effect of hydrogen on pyroptosis in lung grafts remains unclear. In this study, we will assess the effects of lung inflation with 3% hydrogen during the CIP on pyroptosis in lung grafts.<\/p>\n<\/div>\n<div id=\"S2\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S2title\">Materials and Methods<\/h2>\n<div id=\"S2.SS1\" class=\"sec sec-first\">\n<h3 id=\"S2.SS1title\">Study Design<\/h3>\n<p class=\"p p-first\">Adult male pathogen-free Wistar rats weighing 280 \u00b1 11 g were purchased from the Second Affiliated Hospital of Harbin Medical University (Harbin, China) and were housed in individual cages with free access to food and water. The cages were placed in a temperature-controlled room with a 12 h light-dark cycle. All protocols in this study were approved by the Institutional Animal Care and Use Committee of Harbin Medical University.<\/p>\n<p class=\"p\">Fifty-six rats were randomly divided into sham group, the control group, the oxygen (O<sub>2<\/sub>) group, and the hydrogen (H<sub>2<\/sub>) group. After being harvested from donor rats, donor lungs for the control group were deflated, and those for the O<sub>2<\/sub> group and the H<sub>2<\/sub> group were inflated with 40% O<sub>2<\/sub> + 60% N<sub>2<\/sub> and 3% H<sub>2<\/sub> + 40% O<sub>2<\/sub> + 57% N<sub>2<\/sub>, respectively, at 10 ml\/kg (LiMing Gas Corporation, Harbin, China) during the cold storage phase. The inflation gas was replaced every 20 min with an airtight injector (Agilent Corporation, California, United States) for 2 h. Then, the animals in the control group, O<sub>2<\/sub> group, and H<sub>2<\/sub> group underwent lung transplantation. The sham group underwent thoracotomy but no lung transplantation (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F1\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F1\" rid-ob=\"ob-F1\" co-legend-rid=\"lgnd_F1\" rel=\"noopener\"><span>Figure 1<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F1\" co-legend-rid=\"lgnd_F1\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F1\/\" target=\"figure\" rid-figpopup=\"F1\" rid-ob=\"ob-F1\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623274974944\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F1\/\" target=\"figure\" rid-figpopup=\"F1\" rid-ob=\"ob-F1\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g001.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g001.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g001.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623274974944\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F1\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F1\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F1\/\" target=\"figure\" rid-figpopup=\"F1\" rid-ob=\"ob-F1\" rel=\"noopener\">FIGURE 1<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>Study design. The control group, natural collapsed donor lung; The 0<sub>2<\/sub> group, donor lung inflation with 40% oxygen; The H<sub>2<\/sub> group, donor lung inflation with 40% oxygen and 3% hydrogen; CIP, cold ischemia phase; LTx, lung transplantation.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S2.SS2\" class=\"sec\">\n<h3 id=\"S2.SS2title\">Donor Harvesting<\/h3>\n<p class=\"p p-first-last\">The donor rats were anesthetized by intraperitoneal injection of 60 mg\/kg sodium pentobarbital, intubated by tracheotomy, and ventilated with room air (about 21% oxygen) with a tidal volume of 10 ml\/kg (volume-control ventilation mode) and a rate of 40\u201360 breaths\/min and PEEP was 0 (Model 683, Harvard Apparatus, MA, United States). Five minutes after intravenous injection of sodium heparin (1,000 U\/kg) via the tail vein, the donor rats underwent thoracotomy, and the lungs were flushed from the right ventricle with 20 ml low-potassium dextran (LPD) solution prepared by Harbin Medical University according to a previous study (<a href=\"#B22\" rid=\"B22\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Pizanis et al., 2012<\/a>) at a pressure of 20 cmH<sub>2<\/sub>O. Then, the donor lungs were harvested and stored at 4\u00b0C for appropriate ventilation treatment.<\/p>\n<\/div>\n<div id=\"S2.SS3\" class=\"sec\">\n<h3 id=\"S2.SS3title\">Orthotopic Left LTx<\/h3>\n<p class=\"p p-first-last\">The recipient rats were anesthetized and ventilated in a similar manner as the donors. A left thoracotomy was performed in the fourth intercostal space. The hilar structure of the donor lung was fully exposed under a microscope. The tidal volume was adjusted to two-thirds of that before transplantation, and then LTx was performed as described previously (<a href=\"#B32\" rid=\"B32\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Zhai et al., 2008<\/a>). The tidal volume was restored, and 2 cmH<sub>2<\/sub>O positive end-expiratory pressure (PEEP) was applied after transplantation. The recipient rats were observed for 2 h. The breathing rate was adjusted to keep arterial carbon dioxide tension (PaCO<sub>2<\/sub>) within 35\u201345 mmHg. Anesthesia was maintained by intermittent intraperitoneal injection of pentobarbital sodium, muscle relaxation was maintained by pipecuronium bromide (0.4 mg.kg<sup>\u20131<\/sup>.h<sup>\u20131<\/sup>), and humoral balance was maintained by intravenous injection of saline (10 ml.kg<sup>\u20131<\/sup>.h<sup>\u20131<\/sup>). At the end of the experiment, the recipient rats were sacrificed by exsanguination, and the lung grafts were harvested and stored at \u221280\u00b0C for further analysis.<\/p>\n<\/div>\n<div id=\"S2.SS4\" class=\"sec\">\n<h3 id=\"S2.SS4title\">Blood Gas Analysis<\/h3>\n<p class=\"p p-first-last\">Arterial blood gas analysis was performed before transplantation (T<sub>0<\/sub>) and 3 min (T<sub>1<\/sub>), 30 min (T<sub>2<\/sub>), 60 min (T<sub>3<\/sub>), 90 min (T<sub>4<\/sub>), and 120 min (T<sub>5<\/sub>) after reperfusion (Rapid Lab 348, Bayer, Medfield, United States). At the end of the experiment, pulmonary vein blood was collected for blood gas analysis.<\/p>\n<\/div>\n<div id=\"S2.SS5\" class=\"sec\">\n<h3 id=\"S2.SS5title\">Measurement of the Static Compliance of Lung Grafts<\/h3>\n<p class=\"p p-first-last\">Thoracotomy was performed immediately after sacrifice, and the lungs were connected to a homemade apparatus. The static pressure-volume (P-V) curves of the lung grafts were measured to assess static compliance. The airway pressure was measured by increasing the pressure to 30 cmH<sub>2<\/sub>O and then decreasing it to 0 cmH<sub>2<\/sub>O in 5-cm stepwise intervals to evaluate lung volume, and the values were recorded after 30 s of stabilization (<a href=\"#B15\" rid=\"B15\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Meng et al., 2016<\/a>).<\/p>\n<\/div>\n<div id=\"S2.SS6\" class=\"sec\">\n<h3 id=\"S2.SS6title\">Histopathologic Analysis of Lung Grafts<\/h3>\n<p class=\"p p-first-last\">The lung grafts were fixed in 4% paraformaldehyde, embedded in paraffin, cut into 4-\u03bcm thick sections, and stained with hematoxylin and eosin. The parameters that were evaluated included neutrophil infiltration, hemorrhage, interstitial edema, hyaline membrane formation, and airway epithelial cell damage (normal = 0, minimal change = 1, mild change = 2, moderate change = 3, and severe change = 4) (<a href=\"#B21\" rid=\"B21\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Pirat et al., 2006<\/a>). A professional pathologist who was blinded to the study design evaluated all the sections and recorded the lung injury score (LIS).<\/p>\n<\/div>\n<div id=\"S2.SS7\" class=\"sec\">\n<h3 id=\"S2.SS7title\">Measurement of Inflammatory Cytokines Levels in Serum and Oxidative Stress in the Graft<\/h3>\n<p class=\"p p-first-last\">The upper section of the lung graft was placed in an 80\u00b0C oven for approximately 72 h until its weight stabilized, and the wet weight\/dry weight (W\/D) was measured. Myeloperoxidase (MPO) activity, the malondialdehyde (MDA) level, and superoxide dismutase (SOD) activity in the inferior lung graft were determined by respective kits (Jiancheng Bio-Technology, Nanjing, China). The levels of interleukin (IL)-1\u03b2 and IL-18 in serum were measured with enzyme-linked immunosorbent assay (ELISA) kits (Wuhan Boster Bio-Engineering, Co., Ltd., Wuhan, China). The analysis was performed according to the manufacturer\u2019s instructions.<\/p>\n<\/div>\n<div id=\"S2.SS8\" class=\"sec\">\n<h3 id=\"S2.SS8title\">Analysis of Reactive Oxygen Species (ROS) Content in Lung Grafts<\/h3>\n<p class=\"p p-first-last\">A portion of each lung graft was embedded in an optimal cutting temperature compound. Sections (8 \u03bcm thick) were obtained with a freezing microtome (Microm HR560, Thermo Fisher Scientific, United States). The sections were incubated for 30 min with 10 \u03bcM dihydroethidium (DHE) (Beyotime Biotechnology, Shanghai, China) to evaluate lung superoxide anion levels <em>in situ<\/em>. DHE was oxidized by superoxide anion to yield ethidium, which stains DNA with bright red fluorescence. Images were taken with a fluorescence microscope (OLYMPUS BX53M, Olympus Corporation, Japan). The fluorescence intensity was quantified by ImageJ software (v1.33, NIH, Bethesda, MD, United States).<\/p>\n<\/div>\n<div id=\"S2.SS9\" class=\"sec\">\n<h3 id=\"S2.SS9title\">Detection of Pyroptosis-Related Proteins<\/h3>\n<p class=\"p p-first-last\">Total proteins were extracted from lung tissue homogenates using RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China). The total protein concentrations were measured using an enhanced BCA protein assay kit (Beyotime Biotechnology, Shanghai, China) according to the manufacturer\u2019s instructions. Subsequently, the extracted protein samples were separated on 10\u201312.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). After electrophoresis, the proteins were transferred to polyvinylidene fluoride (PVDF) membranes, and the PVDF membranes were blocked with 5% fat-free milk for 2 h. Then, the membranes were incubated with primary antibodies against NLRP3 (1:1,000, Abcam, Cambridge, United Kingdom), precursors of cysteinyl aspartate specific proteinase (procaspase)-1(1:500, Wanleibio, Shenyang, China) and cysteinyl aspartate specific proteinase (caspase-1) p20 (1:500, Wanleibio, Shenyang, China), gasdermin D (GSDMD), the N-terminal of GSDMD (GSDMD-N) (1:1,000, Cell Signaling Technology, United States), and \u03b2-actin (1:1,000, ZSGB-BIO, Beijing, China) at 4\u00b0C for 24 h. After three washes, the membranes were incubated with a peroxidase-conjugated secondary antibody (1:1,000, ZSGB-BIO, Beijing, China). Finally, the immune complexes were visualized with BeyoECL Plus (Beyotime Biotechnology, Shanghai, China), and the bands were quantified with ImageJ software.<\/p>\n<\/div>\n<div id=\"S2.SS10\" class=\"sec\">\n<h3 id=\"S2.SS10title\">Transmission Electron Microscopy (TEM) Analysis of Type II Alveolar Epithelial Cell Morphology<\/h3>\n<p class=\"p p-first-last\">Pieces of lung grafts (1 mm<sup>3<\/sup>) were collected and fixed in 2.5% glutaraldehyde to observe the morphology of cytoplasm and nucleus. The samples were tested by a specialist at the Electron Microscope Center of Harbin Medical University who was blinded to the study design.<\/p>\n<\/div>\n<div id=\"S2.SS11\" class=\"sec sec-last\">\n<h3 id=\"S2.SS11title\">Statistical Analysis<\/h3>\n<p class=\"p p-first-last\">SPSS 21.0 statistical software was used for analysis, and the data are expressed as the mean values \u00b1 standard deviations or medians (interquartile ranges). Group comparisons for normal data were performed by one-way analysis of variance (ANOVA) followed by the Student-Newman-Keuls test or the Kruskal-Wallis test. Repeated measurement data were analyzed by repeated-measures ANOVA. Differences were considered statistically significant at <em>P<\/em> &lt; 0.05.<\/p>\n<\/div>\n<\/div>\n<div id=\"S3\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S3title\">Results<\/h2>\n<div id=\"S3.SS1\" class=\"sec sec-first\">\n<h3 id=\"S3.SS1title\">Recipient Blood Gas Analysis<\/h3>\n<p class=\"p p-first\">In the sham group, partial pressure of arterial oxygen (PaO<sub>2<\/sub>)\/fraction of inspired oxygen (FiO<sub>2<\/sub>) was stable. At T<sub>5<\/sub>, the PaO<sub>2<\/sub>\/FiO<sub>2<\/sub> of the control group (297 \u00b1 33 mmHg) was lower than that of the sham group (444 \u00b1 13 mmHg) (<em>P<\/em> &lt; 0.05). The PaO<sub>2<\/sub>\/FiO<sub>2<\/sub> of the O<sub>2<\/sub> (339 \u00b1 27 mmHg) and H<sub>2<\/sub> (382 \u00b1 23 mmHg) groups was higher than that of the control group (<em>P<\/em> &lt; 0.05). Furthermore, the PaO<sub>2<\/sub>\/FiO<sub>2<\/sub> of the H<sub>2<\/sub> group was higher than that of the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05) (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F2\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F2\" rid-ob=\"ob-F2\" co-legend-rid=\"lgnd_F2\" rel=\"noopener\"><span>Figure 2<\/span><\/a>). Besides, pulmonary venous oxygen tension (PvO<sub>2<\/sub>)\/FiO<sub>2<\/sub> which is more directly reflects the pulmonary ventilation function exhibited the same trend as the PaO<sub>2<\/sub>\/FiO<sub>2<\/sub> (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/table\/T1\/\" target=\"table\" class=\"fig-table-link figpopup\" rid-figpopup=\"T1\" rid-ob=\"ob-T1\" co-legend-rid=\"\" rel=\"noopener\"><span>Table 1<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F2\" co-legend-rid=\"lgnd_F2\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F2\/\" target=\"figure\" rid-figpopup=\"F2\" rid-ob=\"ob-F2\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623276779168\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F2\/\" target=\"figure\" rid-figpopup=\"F2\" rid-ob=\"ob-F2\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g002.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g002.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g002.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623276779168\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F2\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F2\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F2\/\" target=\"figure\" rid-figpopup=\"F2\" rid-ob=\"ob-F2\" rel=\"noopener\">FIGURE 2<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>The indices of PaO<sub>2<\/sub>\/FiO<sub>2<\/sub> in each group (<em>n<\/em> = 8). Values are mean values \u00b1 standard deviations. T<sub>0<\/sub>\u2013T<sub>5<\/sub> represented the following time points: baseline before transplantation, and 3, 30, 60, 90, and 120 min after reperfusion. PaO<sub>2<\/sub>\/FiO<sub>2<\/sub>, partial pressure of arterial oxygen (PaO<sub>2<\/sub>)\/fraction of inspired oxygen (FiO<sub>2<\/sub>). <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<p><!--table ft1--><!--table-wrap mode=\"anchored\" t5--><\/p>\n<div class=\"table-wrap anchored whole_rhythm\" id=\"T1\">\n<h3>TABLE 1<\/h3>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>PvO<sub>2<\/sub>\/FiO<sub>2<\/sub> and the indices of the inflammatory response and oxidative stress in each group (mean \u00b1 SD, <em>n<\/em> = 8).<\/p>\n<\/div>\n<div class=\"xtable\">\n<table frame=\"hsides\" rules=\"groups\" cellspacing=\"5\" cellpadding=\"5\" class=\"rendered small default_table\">\n<thead>\n<tr>\n<td valign=\"top\" align=\"left\" rowspan=\"1\" colspan=\"1\"><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>PvO<sub>2<\/sub>\/FiO<sub>2<\/sub> (mmHg)<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>W\/D<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>MPO (U\/g)<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>IL-1\u03b2 (pg\/ml)<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>IL-18 (pg\/ml)<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>MDA (nmol\/mg prot)<\/strong>\n<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">\n<strong>SOD (U\/mg prot)<\/strong>\n<\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td valign=\"top\" align=\"left\" rowspan=\"1\" colspan=\"1\">Sham<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">448 \u00b1 15<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">4.5 \u00b1 0.3<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">0.47 \u00b1 0.14<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">47 \u00b1 16<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">15 \u00b1 5<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">0.79 \u00b1 0.27<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">182 \u00b1 36<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" align=\"left\" rowspan=\"1\" colspan=\"1\">Control<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">299 \u00b1 41*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">6.8 \u00b1 1.1*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">1.18 \u00b1 0.28*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">219 \u00b1 51*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">112 \u00b1 29*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">2.50 \u00b1 0.52*<\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">82 \u00b1 26*<\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" align=\"left\" rowspan=\"1\" colspan=\"1\">O<sub>2<\/sub><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">348 \u00b1 26*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">5.7 \u00b1 0.8*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">0.94 \u00b1 0.20*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">151 \u00b1 37*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">74 \u00b1 18*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">1.67 \u00b1 0.47*<sup>#<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">129 \u00b1 43*<sup>#<\/sup><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\" align=\"left\" rowspan=\"1\" colspan=\"1\">H<sub>2<\/sub><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">395 \u00b1 32*<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">4.9 \u00b1 0.6<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">0.61 \u00b1 0.18<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">90 \u00b1 26*<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">44 \u00b1 12*<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">0.95 \u00b1 0.38<sup>#\u25b3<\/sup><\/td>\n<td valign=\"top\" align=\"center\" rowspan=\"1\" colspan=\"1\">167 \u00b1 34<sup>#\u25b3<\/sup><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623276775328\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/table\/T1\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"tblwrap-foot\">\n<div class=\"half_rhythm align_right attrib\"><em>PvO<sub>2<\/sub>\/FiO<sub>2<\/sub>, pulmonary venous oxygen tension (PaO<sub>2<\/sub>)\/fraction of inspired oxygen (FiO<sub>2<\/sub&gt;);IL, Interleukin; MDA, malonaldehyde; MPO, myeloperoxidase; SOD, superoxide dismutase W\/D, wet weight (W)\/dry weight (D). *P &lt; 0.05 vs. sham group; <sup>#<\/sup>P &lt; 0.05 vs. control group; <sup>\u25b3<\/sup>P &lt; 0.05 vs. O<sub>2<\/sub> group.<\/em><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S3.SS2\" class=\"sec\">\n<h3 id=\"S3.SS2title\">The P-V Curves of the Lung Grafts<\/h3>\n<p class=\"p p-first\">At a pressure of 30 cmH<sub>2<\/sub>O, the P-V curve values of the control group (11.80 \u00b1 0.26 ml\/kg) were lower than those of the sham group (21.37 \u00b1 1.00 ml\/kg) (<em>P<\/em> &lt; 0.05). The P-V curve values of the O<sub>2<\/sub> group (14.50 \u00b1 0.53 ml\/kg) and the H<sub>2<\/sub> group (17.40 \u00b1 0.26 ml\/kg) were higher than those of the control group (<em>P<\/em> &lt; 0.05). The P-V curve values of the H<sub>2<\/sub> group were significantly higher than those of the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05) (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F3\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F3\" rid-ob=\"ob-F3\" co-legend-rid=\"lgnd_F3\" rel=\"noopener\"><span>Figure 3<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F3\" co-legend-rid=\"lgnd_F3\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F3\/\" target=\"figure\" rid-figpopup=\"F3\" rid-ob=\"ob-F3\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623240054976\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F3\/\" target=\"figure\" rid-figpopup=\"F3\" rid-ob=\"ob-F3\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g003.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g003.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g003.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623240054976\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F3\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F3\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F3\/\" target=\"figure\" rid-figpopup=\"F3\" rid-ob=\"ob-F3\" rel=\"noopener\">FIGURE 3<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>The pressure-volume (P\u2013V) curve in the lung graft (<em>n<\/em> = 3). Values are means, standard deviation bars were not shown for clarity. At a pressure of 30 cmH<sub>2<\/sub>O, the lung volume in the control group was lower than the sham group (<em>P<\/em> &lt; 0.05), the lung volume in O<sub>2<\/sub> and H<sub>2<\/sub> groups was higher than the control group (<em>P<\/em> &lt; 0.05), the lung volume in the H<sub>2<\/sub> group was higher than the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S3.SS3\" class=\"sec\">\n<h3 id=\"S3.SS3title\">The Inflammatory Response in the Recipients<\/h3>\n<p class=\"p p-first-last\">The W\/D ratio of the graft in the control group (6.8 \u00b1 1.1) was higher than that of the sham group (4.5 \u00b1 0.3) (<em>P<\/em> &lt; 0.05). The W\/D ratio of the graft in the O<sub>2<\/sub> group (5.7 \u00b1 0.8) and H<sub>2<\/sub> group (4.9 \u00b1 0.6) was lower than that of the control group (<em>P<\/em> &lt; 0.05). The W\/D ratio of the graft in the H<sub>2<\/sub> group was lower than that of the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). MPO activity in the grafts and the levels of IL-1\u03b2 and IL-18 in the serum exhibited a similar tendency as the W\/D ratio (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/table\/T1\/\" target=\"table\" class=\"fig-table-link figpopup\" rid-figpopup=\"T1\" rid-ob=\"ob-T1\" co-legend-rid=\"\" rel=\"noopener\"><span>Table 1<\/span><\/a>).<\/p>\n<\/div>\n<div id=\"S3.SS4\" class=\"sec\">\n<h3 id=\"S3.SS4title\">The Oxidative Stress Response in the Recipients<\/h3>\n<p class=\"p p-first\">SOD activity in the graft in the control group (82 \u00b1 26 U\/mg protein) was lower than that in the sham group (182 \u00b1 36 U\/mg protein) (<em>P<\/em> &lt; 0.05). SOD activity in the graft in the O<sub>2<\/sub> group (129 \u00b1 43 U\/mg protein) and the H<sub>2<\/sub> group (167 \u00b1 34 U\/mg protein) was higher than that in the control group (<em>P<\/em> &lt; 0.05). SOD activity in the H<sub>2<\/sub> group was higher than that in the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). MDA levels in the graft exhibited the opposite tendency as SOD activity (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/table\/T1\/\" target=\"table\" class=\"fig-table-link figpopup\" rid-figpopup=\"T1\" rid-ob=\"ob-T1\" co-legend-rid=\"\" rel=\"noopener\"><span>Table 1<\/span><\/a>).<\/p>\n<p class=\"p\">Similarly, the ROS level in the control group (43.2 \u00b1 7.2) was higher than that in the sham group (17.7 \u00b1 0.9), the ROS level in the O<sub>2<\/sub> group (34.1 \u00b1 4.4), and H<sub>2<\/sub> group (24.1 \u00b1 2.5) was lower than that in the control group, and the H<sub>2<\/sub> group produced fewer ROS than the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05) (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F4\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F4\" rid-ob=\"ob-F4\" co-legend-rid=\"lgnd_F4\" rel=\"noopener\"><span>Figure 4<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F4\" co-legend-rid=\"lgnd_F4\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F4\/\" target=\"figure\" rid-figpopup=\"F4\" rid-ob=\"ob-F4\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623239532032\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F4\/\" target=\"figure\" rid-figpopup=\"F4\" rid-ob=\"ob-F4\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g004.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g004.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g004.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623239532032\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F4\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F4\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F4\/\" target=\"figure\" rid-figpopup=\"F4\" rid-ob=\"ob-F4\" rel=\"noopener\">FIGURE 4<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>The ROS stained with dihydroethidium imaged by fluorescence microscope. (<em>n<\/em> = 3, original magnification, 20) <strong>(A)<\/strong> sham group; <strong>(B)<\/strong> control group; <strong>(C)<\/strong> O<sub>2<\/sub> group; <strong>(D)<\/strong> H<sub>2<\/sub> group; <strong>(E)<\/strong> the ROS levels in lung grafts. The ROS level in the control group was higher than the sham group (<em>P<\/em> &lt; 0.05), the ROS levels in the O<sub>2<\/sub> and H<sub>2<\/sub> group were lower than the control group (<em>P<\/em> &lt; 0.05), the H<sub>2<\/sub> group was lower than the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S3.SS5\" class=\"sec\">\n<h3 id=\"S3.SS5title\">Recipient Lung Injury Scores<\/h3>\n<p class=\"p p-first\">The lung grafts were almost normal in the sham group, and few pathological changes were found. However, numerous pathological changes were found in the control group, including severe interstitial edema, intra-alveolar hemorrhage, and massive neutrophil infiltration. There were fewer changes in the grafts in the O<sub>2<\/sub> group and H<sub>2<\/sub> group than in the control group, and hydrogen had a better therapeutic effect than O<sub>2<\/sub>. The LIS related to neutrophil infiltration in the control group [3.5(3\u20134)] was higher than that in the sham group [0.5(0\u20131)] (<em>P<\/em> &lt; 0.05). The LIS related to neutrophil infiltration in the O2 group [2(1.75\u20132.25)] and H2 group [1(0.75\u20132)] was lower than that in the control group, but the difference between the control group and the O2 group was not significant. The LIS of the H2 group was lower than that of the O2 group, but the difference was not significant. Similarly, the LISs related to other criteria exhibited a similar trend as the LIS related to neutrophil infiltration (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F5\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F5\" rid-ob=\"ob-F5\" co-legend-rid=\"lgnd_F5\" rel=\"noopener\"><span>Figure 5<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F5\" co-legend-rid=\"lgnd_F5\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F5\/\" target=\"figure\" rid-figpopup=\"F5\" rid-ob=\"ob-F5\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623239517024\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F5\/\" target=\"figure\" rid-figpopup=\"F5\" rid-ob=\"ob-F5\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g005.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g005.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g005.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623239517024\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F5\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F5\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F5\/\" target=\"figure\" rid-figpopup=\"F5\" rid-ob=\"ob-F5\" rel=\"noopener\">FIGURE 5<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>Histologic analyses of lung grafts (<em>n<\/em> = 8, original magnification, 40). <strong>(A)<\/strong> sham group; <strong>(B)<\/strong> control group; <strong>(C)<\/strong> O<sub>2<\/sub> group; <strong>(D)<\/strong> H<sub>2<\/sub> group; <strong>(E)<\/strong> Lung injury score (LIS). The LIS in the control group was higher than the sham group (<em>P<\/em> &lt; 0.05), the H<sub>2<\/sub> group was lower than the control group (<em>P<\/em> &lt; 0.05). <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S3.SS6\" class=\"sec\">\n<h3 id=\"S3.SS6title\">Pyroptosis-Related Data in the Recipients<\/h3>\n<p class=\"p p-first\">The levels of NLRP3 in the lung grafts were higher in the control group (2.5 \u00b1 0.8) than that in the sham group (<em>P<\/em> &lt; 0.05). The levels of NLRP3 in the lung grafts in the O<sub>2<\/sub> group (2.4 \u00b1 0.7) and H<sub>2<\/sub> group (1.3 \u00b1 0.3) were lower than those in the lung grafts in the control group, but the difference between the control group and the O<sub>2<\/sub> group was not significant. In the H<sub>2<\/sub> group, the level of NLRP3 was lower than that in the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). The levels of caspase-1 p20 and GSDMD-N exhibited a similar tendency as the level of NLRP3. The levels of procaspase-1 and GSDMD were not different between the groups (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F6\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F6\" rid-ob=\"ob-F6\" co-legend-rid=\"lgnd_F6\" rel=\"noopener\"><span>Figure 6<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F6\" co-legend-rid=\"lgnd_F6\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F6\/\" target=\"figure\" rid-figpopup=\"F6\" rid-ob=\"ob-F6\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623234689024\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F6\/\" target=\"figure\" rid-figpopup=\"F6\" rid-ob=\"ob-F6\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g006.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g006.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g006.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623234689024\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F6\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F6\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F6\/\" target=\"figure\" rid-figpopup=\"F6\" rid-ob=\"ob-F6\" rel=\"noopener\">FIGURE 6<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>The protein expressions in the lung graft (<em>n<\/em> = 3). <strong>(A)<\/strong> Representative western blots and semiquantitative analysis of caspase-1 p20 <strong>(B)<\/strong>, NLRP3 <strong>(C)<\/strong>, GSDMD-N <strong>(D)<\/strong>. The expressions of caspase-1 p20, NLRP3, and GSDMD-N in the control group were higher than the sham group (<em>P<\/em> &lt; 0.05), Caspase-1 p20, NLRP3, and GSDMD-N in the O<sub>2<\/sub> group were lower than the control group, but the difference of NLRP3 and GSDMD-N had no statistically significant, Caspase-1 p20, NLRP3, and GSDMD-N in the H<sub>2<\/sub> group were lower than the control group and the O<sub>2<\/sub> group (<em>P<\/em> &lt; 0.05). <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S3.SS7\" class=\"sec sec-last\">\n<h3 id=\"S3.SS7title\">Electron Microscopy Analysis of Morphological Changes in Type II Alveolar Epithelial Cells<\/h3>\n<p class=\"p p-first\">The ultrastructural changes in the lung grafts were observed by TEM. In the sham group, the type II alveolar epithelial cells were almost normal. However, severely swollen cytoplasmic and nuclear pyknosis was found in the control group, there were fewer changes in the O<sub>2<\/sub> group than in the control group, and few changes were found in the H<sub>2<\/sub> group (<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F7\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"F7\" rid-ob=\"ob-F7\" co-legend-rid=\"lgnd_F7\" rel=\"noopener\"><span>Figure 7<\/span><\/a>).<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"F7\" co-legend-rid=\"lgnd_F7\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F7\/\" target=\"figure\" rid-figpopup=\"F7\" rid-ob=\"ob-F7\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/a><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140623236819360\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F7\/\" target=\"figure\" rid-figpopup=\"F7\" rid-ob=\"ob-F7\" rel=\"noopener\"><\/a><a class=\"inline_block ts_canvas\" href=\"\/core\/lw\/2.0\/html\/tileshop_pmc\/tileshop_pmc_inline.html?title=Click%20on%20image%20to%20zoom&amp;p=PMC3&amp;id=8365359_fphys-12-699344-g007.jpg\" target=\"tileshopwindow\" rel=\"noopener\"><\/p>\n<div class=\"ts_bar small\" title=\"Click on image to zoom\"><\/div>\n<p><img decoding=\"async\" loading=\"lazy\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is fphys-12-699344-g007.jpg\" title=\"Click on image to zoom\" class=\"tileshop\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/fphys-12-699344-g007.jpg\"><\/a><\/div>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140623236819360\" style=\"display: none;\"><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F7\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_F7\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/figure\/F7\/\" target=\"figure\" rid-figpopup=\"F7\" rid-ob=\"ob-F7\" rel=\"noopener\">FIGURE 7<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>Type II alveolar epithelial cell morphology was imaged with electron microscopy (original magnification, 10,000). <strong>(A)<\/strong> sham group; <strong>(B)<\/strong> control group; <strong>(C)<\/strong> O<sub>2<\/sub> group; <strong>(D)<\/strong> H<sub>2<\/sub> group. The type II alveolar epithelial cell morphology was normal in the sham group, the swollen cell and nuclear pyknosis were observed in the control group, the changes were improved in the O<sub>2<\/sub> group, the cell had fewer changes in the H<sub>2<\/sub> group. <sup>\u2217<\/sup><em>P<\/em> &lt; 0.05 vs. sham group; <sup>#<\/sup><em>P<\/em> &lt; 0.05 vs. control group; <sup>\u25b3<\/sup><em>P<\/em> &lt; 0.05 vs. O<sub>2<\/sub> group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"S4\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S4title\">Discussion<\/h2>\n<p class=\"p p-first\">In this study, the expression of pyroptosis-related proteins, including NLRP3, caspase-1 p20, and GSDMD-N, was increased, and pyroptotic type II alveolar epithelial cells were found in lung grafts by electron microscopy. These data indicate that pyroptosis occurred in lung grafts. When the donor lung was inflated with 40% oxygen during the CIP, oxygenation and static compliance were improved, and oxidative stress indices, including MDA levels and SOD activity, were improved. Inflammatory response indices, including the W\/D ratio, MPO activity, and the levels of IL-1\u03b2 and IL-18, were decreased. The morphology of type II alveolar epithelial cells was improved, and the expression of caspase-1 p20 was decreased, which is the result of the combination of oxygen and lung inflation. When oxygen was combined with 3% hydrogen, the above indices related to the inflammatory response and oxidative stress were further improved, the expression of caspase-1 p20, NLRP3, and GSDMD-N was decreased.<\/p>\n<p>In donor lungs, the application of hydrogen in the cold storage phase includes lung inflation and the use of hydrogen-rich preservation solution (<a href=\"#B22\" rid=\"B22\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Pizanis et al., 2012<\/a&gt;; <a href=\"#B31\" rid=\"B31\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Xu et al., 2018<\/a>). <a href=\"#B11\" rid=\"B11\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Liu et al. (2015)<\/a> found that lung inflation with 3% hydrogen during the cold ischemia phase alleviated lung graft injury. <a href=\"#B28\" rid=\"B28\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Takahashi et al. (2017)<\/a> found that immersing lungs of rats in hydrogen-rich saline attenuated lung ischemia-reperfusion injury (IRI) by decreasing inflammatory cytokine levels and improving pulmonary function. Compared with the use of hydrogen-rich preservation solution, lung inflation with hydrogen is simpler and easier and does not require additional equipment. The optimal concentration of hydrogen has not been determined. <a href=\"#B12\" rid=\"B12\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Liu et al. (2017)<\/a> found that inhalation of 22 and 41.6% hydrogen had a better therapeutic effect on chronic obstructive pulmonary disease. Chen X found that the protective effects of 55\u201375% hydrogen on <em>in vitro<\/em> neuronal mechanical injury were dose-dependent (<a href=\"#B1\" rid=\"B1\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Chen et al., 2018<\/a>). In this study, 3% hydrogen was chosen because of its safety, and because it could alleviate lung IRI in our previous experiments (<a href=\"#B11\" rid=\"B11\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Liu et al., 2015<\/a>).<\/p>\n<p>Lung inflation is an effective method for preserving the donor lung. <a href=\"#B8\" rid=\"B8\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Kao et al. (2004)<\/a> found that static lung inflation with a mixture of room air and 5% CO<sub>2<\/sub> attenuated IRI by decreasing the capillary filtration coefficient and protein concentration in bronchoalveolar lavage fluid. <a href=\"#B7\" rid=\"B7\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fukuse et al. (1999)<\/a> found that lung inflation with room air exerted protective effects through both lung inflation and oxygen. Inflation of the alveoli can prevent mechanical damage during reexpansion, and oxygen can attenuate lung injury through maintaining aerobic metabolism (<a href=\"#B7\" rid=\"B7\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fukuse et al., 1999<\/a>). <a href=\"#B30\" rid=\"B30\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Weder et al. (1991)<\/a> thought that lung preservation with 100% oxygen inflation appears superior to inflation with room air. However, <a href=\"#B6\" rid=\"B6\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fukuse et al. (2001)<\/a> thought hyperoxygenation (50 or 100% oxygen) induced mitochondrial dysfunction and increased lipid peroxidation. Therefore, 40% oxygen was chosen for lung inflation in the oxygen group. To further improve the treatment of lung inflation with 40% oxygen, 3% hydrogen was added on 40% oxygen (<a href=\"#B6\" rid=\"B6\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fukuse et al., 2001<\/a>). Compared with lung inflation with toxic hydrogen sulfide and carbon monoxide, lung inflation with 3% hydrogen is safer and less harmful (<a href=\"#B6\" rid=\"B6\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fukuse et al., 2001<\/a>). In this study, compared with natural collapse of the lung, lung inflation with 40% O<sub>2<\/sub> improved lung graft function, decreased the inflammatory response and oxidative stress, and attenuated lung IRI. Lung IRI was further ameliorated in the hydrogen group, indicating that hydrogen strengthened the protective effect of lung inflation with 40% O<sub>2<\/sub>, which is consistent with our previous studies (<a href=\"#B11\" rid=\"B11\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Liu et al., 2015<\/a>).<\/p>\n<p>Severe IRI can lead to primary graft dysfunction. Oxidative stress and the inflammatory response play an important role in lung IRI (<a href=\"#B3\" rid=\"B3\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">de Perrot et al., 2003<\/a&gt;; <a href=\"#B16\" rid=\"B16\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Ng et al., 2006<\/a>). Hydrogen has anti-inflammatory and selective antioxidant effects. <a href=\"#B9\" rid=\"B9\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Kawamura et al. (2010)<\/a> found that inhaled hydrogen inhibited the production of proinflammatory mediators and reduced graft lipid peroxidation. In this experiment, lung inflation with hydrogen during the CIP decreased inflammatory indices, including the levels of IL-1\u03b2 and IL-18 in the serum, the W\/D ratio, and MPO activity in grafts, and inhibited oxidative stress, including by increasing SOD activity and reducing MDA and ROS levels. Our previous experiment also revealed that lung inflation with hydrogen could inhibit the inflammatory factors TNF-\u03b1 and IL-8 (<a href=\"#B11\" rid=\"B11\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Liu et al., 2015<\/a>). These protective effects of hydrogen might be achieved through inhibition of the p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-\u03baB) signaling pathways (<a href=\"#B33\" rid=\"B33\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Zhang et al., 2018<\/a>).<\/p>\n<p>Pyroptosis is a form of inflammatory programmed cell death mediated by GSDMD. Moderate pyroptosis can act as a self-protective mechanism against infection, and severe pyroptosis can aggravate lung injury (<a href=\"#B14\" rid=\"B14\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Man et al., 2017<\/a>). <a href=\"#B5\" rid=\"B5\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Fei et al. (2020)<\/a> found that severe pyroptosis was present in a mouse model of lung IRI and inhibited pyroptosis via the alveolar macrophage pathway to ameliorate lung IRI with recombinant high-mobility group box 1. <a href=\"#B31\" rid=\"B31\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Xu et al. (2018)<\/a> found that in a mouse model of lung IRI, pyroptosis in the lung was more severe 2 h after reperfusion than 1 or 6 h after reperfusion. Therefore, 2 h of reperfusion was used in this experiment. Electron microscopy and analysis of pyroptosis-related protein expression revealed that NLRP3 inflammasome-mediated pyroptosis was involved in lung IRI.<\/p>\n<p>Ion flux, ROS, and lysosome rupture are the main pathways to activate the NLRP3 inflammasome (<a href=\"#B4\" rid=\"B4\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">de Zoete et al., 2014<\/a>). ROS, as second messengers, are considered important for the activation of the NLRP3 inflammasome. <a href=\"#B23\" rid=\"B23\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Qiu et al. (2017)<\/a> found that ROS activated the NLRP3 inflammasome, which triggered caspase-1-dependent pyroptosis in myocardial IRI in diabetic rats. Some studies have confirmed that hydrogen inhibits NLRP3 inflammasome activation and reduces pyroptosis in the brain and lung injury (<a href=\"#B27\" rid=\"B27\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Shao et al., 2016<\/a&gt;; <a href=\"#B36\" rid=\"B36\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Zou et al., 2019<\/a>). In this experiment, lung inflation with 3% hydrogen during the CIP decreased ROS levels and inhibited pyroptosis-related indices, including the expression of NLRP3, caspase-1 p20, and GSDMD-N. Inhibiting ROS production might be the main mechanism of the antipyroptotic effect of hydrogen. Coincidentally, Ren JD found that molecular hydrogen inhibited lipopolysaccharide-triggered NLRP3 inflammasome activation in macrophages by targeting mitochondrial ROS (<a href=\"#B25\" rid=\"B25\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Ren et al., 2016<\/a>).<\/p>\n<p>This experiment had the following limitations. First, lung inflation for 2 h and reperfusion for 2 h after transplantation might be too short, the long-term effects of hydrogen on lung IRI need to be further studied. Second, we found that pyroptosis occurred in lung grafts 2 h after transplantation. However, the regulatory mechanism of pyroptosis was not elucidated. Third, only the classical NLRP3 inflammasome-mediated pyroptosis pathway was assessed, and other classical and non-classical pyroptosis pathways need to be further studied. Fourth, this study only examined the therapeutic effect of 3% hydrogen, and the effects of high-concentration hydrogen need to be further studied. Fifth, the donor lungs of the control group weren\u2019t inflated. Sixth, reactive oxygen species, the lung compliance, and the expressions of pyroptosis-related proteins were only measured in 3 animals.<\/p>\n<p class=\"p p-last\">In conclusion, pyroptosis occurred in lung grafts, and lung inflation with 3% hydrogen during the cold ischemia phase had anti-inflammatory, antioxidant, and antipyroptotic effects during lung transplantation. Preventing pyroptosis might be one of the mechanisms by which hydrogen alleviates lung IRI. Inhibiting ROS production might be the main mechanism of the antipyroptotic effect of hydrogen.<\/p>\n<\/div>\n<div id=\"S5\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S5title\">Data Availability Statement<\/h2>\n<p class=\"p p-first-last\">The original contributions presented in the study are included in the article\/<a href=\"#TS1\" rid=\"TS1\" class=\" supplementary-material\">Supplementary Material<\/a>, further inquiries can be directed to the corresponding author\/s.<\/p>\n<\/div>\n<div id=\"S6\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S6title\">Ethics Statement<\/h2>\n<p class=\"p p-first-last\">The animal study was reviewed and approved by The Fourth Affiliated Hospital of Harbin Medical University.<\/p>\n<\/div>\n<div id=\"S7\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S7title\">Author Contributions<\/h2>\n<p class=\"p p-first-last\">PZ, JK, and HZ designed the study. PZ, JK, and EX performed the study. XW, BZ, and HZ analyzed the data. PZ drafted the manuscript. JK and HZ revised the manuscript. All authors contributed to the manuscript and approved the submitted version.<\/p>\n<\/div>\n<div id=\"conf1\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"conf1title\">Conflict of Interest<\/h2>\n<p class=\"p p-first-last\">The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<\/p>\n<\/div>\n<div id=\"h15\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"h15title\">Publisher\u2019s Note<\/h2>\n<p class=\"p p-first-last\">All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.<\/p>\n<\/div>\n<div id=\"fn-group-a.o.a\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"fn-group-a.o.atitle\">Footnotes<\/h2>\n<p><!--back\/fn-group--><\/p>\n<div class=\"fm-sec half_rhythm small\">\n<p class=\"fn sec\" id=\"fn-a.o.a.a\">\n<p class=\"p p-first-last\"><strong>Funding.<\/strong> The authors disclosed receipt of the following financial support for the research: The National Nature Science Foundation of China (Grant No. 81570088).<\/p>\n<\/p>\n<\/div>\n<\/div>\n<div id=\"S9\" class=\"tsec bk-sec\"><a id=\"supplementary-material-sec\"><\/a><\/p>\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"S9title\">Supplementary Material<\/h2>\n<p><!--\/article\/back\/sec\/--><\/p>\n<p class=\"p p-first\">The Supplementary Material for this article can be found online at: <a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2021.699344\/full#supplementary-material\" data-ga-action=\"click_feat_suppl\" ref=\"reftype=extlink&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CBody&amp;TO=External%7CLink%7CURI\" target=\"_blank\" rel=\"noopener\">https:\/\/www.frontiersin.org\/articles\/10.3389\/fphys.2021.699344\/full#supplementary-material<\/a><\/p>\n<div class=\"sec suppmat\" id=\"TS1\">\n<div class=\"sup-box half_rhythm\" id=\"media-a.o.b.c.a\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/bin\/Table_1.DOC\" data-ga-action=\"click_feat_suppl\">Click here for additional data file.<\/a><sup>(45K, DOC)<\/sup><\/div>\n<\/div>\n<div class=\"sec suppmat\" id=\"TS2\">\n<div class=\"sup-box half_rhythm\" id=\"media-a.o.b.d.a\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC8365359\/bin\/Table_2.DOC\" data-ga-action=\"click_feat_suppl\">Click here for additional data file.<\/a><sup>(32K, DOC)<\/sup><\/div>\n<\/div>\n<\/div>\n<div id=\"ref-list-a.o.c\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"ref-list-a.o.ctitle\">References<\/h2>\n<div class=\"ref-list-sec sec\" id=\"reference-list\">\n<ul class=\"back-ref-list\" style=\"list-style-type:decimal;\">\n<li id=\"B1\"><span class=\"mixed-citation\">Chen X., Cui J., Zhai X., Zhang J., Gu Z., Zhi X., et al. (2018). <span class=\"ref-title\">Inhalation of hydrogen of different concentrations ameliorates spinal cord injury in mice by protecting spinal cord neurons from apoptosis, oxidative injury and mitochondrial structure damages.<\/span><br \/>\n<span class=\"ref-journal\"><em>Cell Physiol. Biochem.<\/em><\/span><br \/>\n<span class=\"ref-vol\">47<\/span><br \/>\n176\u2013190. 10.1159\/000489764<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29763919\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1159%2F000489764\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Cell+Physiol.+Biochem.&amp;title=Inhalation+of+hydrogen+of+different+concentrations+ameliorates+spinal+cord+injury+in+mice+by+protecting+spinal+cord+neurons+from+apoptosis,+oxidative+injury+and+mitochondrial+structure+damages.&amp;author=X.+Chen&amp;author=J.+Cui&amp;author=X.+Zhai&amp;author=J.+Zhang&amp;author=Z.+Gu&amp;volume=47&amp;publication_year=2018&amp;pages=176-190&amp;pmid=29763919&amp;doi=10.1159\/000489764&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B2\"><span class=\"mixed-citation\">de Perrot M., Keshavjee S. (2004). <span class=\"ref-title\">Lung preservation.<\/span><br \/>\n<span class=\"ref-journal\"><em>Semin. Thorac. Cardiovasc. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">16<\/span><br \/>\n300\u2013308. 10.1053\/j.semtcvs.2004.09.012<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/15635534\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1053%2Fj.semtcvs.2004.09.012\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Semin.+Thorac.+Cardiovasc.+Surg.&amp;title=Lung+preservation.&amp;author=M.+de+Perrot&amp;author=S.+Keshavjee&amp;volume=16&amp;publication_year=2004&amp;pages=300-308&amp;pmid=15635534&amp;doi=10.1053\/j.semtcvs.2004.09.012&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B3\"><span class=\"mixed-citation\">de Perrot M., Liu M., Waddell T. K., Keshavjee S. (2003). <span class=\"ref-title\">Ischemia-reperfusion-induced lung injury.<\/span><br \/>\n<span class=\"ref-journal\"><em>Am. J. Respir. Crit. Care Med.<\/em><\/span><br \/>\n<span class=\"ref-vol\">167<\/span><br \/>\n490\u2013511. 10.1164\/rccm.200207-670SO<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/12588712\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1164%2Frccm.200207-670SO\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Am.+J.+Respir.+Crit.+Care+Med.&amp;title=Ischemia-reperfusion-induced+lung+injury.&amp;author=M.+de+Perrot&amp;author=M.+Liu&amp;author=T.+K.+Waddell&amp;author=S.+Keshavjee&amp;volume=167&amp;publication_year=2003&amp;pages=490-511&amp;pmid=12588712&amp;doi=10.1164\/rccm.200207-670SO&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B4\"><span class=\"mixed-citation\">de Zoete M. R., Palm N. W., Zhu S., Flavell R. A. (2014). <span class=\"ref-title\">Inflammasomes.<\/span><br \/>\n<span class=\"ref-journal\"><em>Cold Spring Harb. Perspect. Biol.<\/em><\/span><br \/>\n<span class=\"ref-vol\">6<\/span>:a016287. 10.1101\/cshperspect.a016287<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4292152\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25324215\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1101%2Fcshperspect.a016287\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Cold+Spring+Harb.+Perspect.+Biol.&amp;title=Inflammasomes.&amp;author=M.+R.+de+Zoete&amp;author=N.+W.+Palm&amp;author=S.+Zhu&amp;author=R.+A.+Flavell&amp;volume=6&amp;publication_year=2014&amp;pages=a016287&amp;pmid=25324215&amp;doi=10.1101\/cshperspect.a016287&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B5\"><span class=\"mixed-citation\">Fei L., Jingyuan X., Fangte L., Huijun D., Liu Y., Ren J., et al. (2020). <span class=\"ref-title\">Preconditioning with rHMGB1 ameliorates lung ischemia-reperfusion injury by inhibiting alveolar macrophage pyroptosis via the Keap1\/Nrf2\/HO-1 signaling pathway.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Transl. Med.<\/em><\/span><br \/>\n<span class=\"ref-vol\">18<\/span>:301. 10.1186\/s12967-020-02467-w<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7405465\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32758258\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1186%2Fs12967-020-02467-w\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Transl.+Med.&amp;title=Preconditioning+with+rHMGB1+ameliorates+lung+ischemia-reperfusion+injury+by+inhibiting+alveolar+macrophage+pyroptosis+via+the+Keap1\/Nrf2\/HO-1+signaling+pathway.&amp;author=L.+Fei&amp;author=X.+Jingyuan&amp;author=L.+Fangte&amp;author=D.+Huijun&amp;author=Y.+Liu&amp;volume=18&amp;publication_year=2020&amp;pages=301&amp;pmid=32758258&amp;doi=10.1186\/s12967-020-02467-w&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B6\"><span class=\"mixed-citation\">Fukuse T., Hirata T., Ishikawa S., Shoji T., Yoshimura T., Chen Q., et al. (2001). <span class=\"ref-title\">Optimal alveolar oxygen concentration for cold storage of the lung.<\/span><br \/>\n<span class=\"ref-journal\"><em>Transplantation<\/em><\/span><br \/>\n<span class=\"ref-vol\">72<\/span><br \/>\n300\u2013304. 10.1097\/00007890-200107270-00024<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/11477357\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2F00007890-200107270-00024\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Transplantation&amp;title=Optimal+alveolar+oxygen+concentration+for+cold+storage+of+the+lung.&amp;author=T.+Fukuse&amp;author=T.+Hirata&amp;author=S.+Ishikawa&amp;author=T.+Shoji&amp;author=T.+Yoshimura&amp;volume=72&amp;publication_year=2001&amp;pages=300-304&amp;pmid=11477357&amp;doi=10.1097\/00007890-200107270-00024&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B7\"><span class=\"mixed-citation\">Fukuse T., Hirata T., Nakamura T., Kawashima M., Hitomi S., Wada H. (1999). <span class=\"ref-title\">Influence of deflated and anaerobic conditions during cold storage on rat lungs.<\/span><br \/>\n<span class=\"ref-journal\"><em>Am. J. Respir. Crit. Care Med.<\/em><\/span><br \/>\n<span class=\"ref-vol\">160<\/span><br \/>\n621\u2013627. 10.1164\/ajrccm.160.2.9809023<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10430738\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1164%2Fajrccm.160.2.9809023\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Am.+J.+Respir.+Crit.+Care+Med.&amp;title=Influence+of+deflated+and+anaerobic+conditions+during+cold+storage+on+rat+lungs.&amp;author=T.+Fukuse&amp;author=T.+Hirata&amp;author=T.+Nakamura&amp;author=M.+Kawashima&amp;author=S.+Hitomi&amp;volume=160&amp;publication_year=1999&amp;pages=621-627&amp;pmid=10430738&amp;doi=10.1164\/ajrccm.160.2.9809023&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B8\"><span class=\"mixed-citation\">Kao S. J., Wang D., Yeh D. Y., Hsu K., Hsu Y. H., Chen H. I. (2004). <span class=\"ref-title\">Static inflation attenuates ischemia\/reperfusion injury in an isolated rat lung in situ.<\/span><br \/>\n<span class=\"ref-journal\"><em>Chest<\/em><\/span><br \/>\n<span class=\"ref-vol\">126<\/span><br \/>\n552\u2013558. 10.1378\/chest.126.2.552<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/15302744\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1378%2Fchest.126.2.552\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Chest&amp;title=Static+inflation+attenuates+ischemia\/reperfusion+injury+in+an+isolated+rat+lung+in+situ.&amp;author=S.+J.+Kao&amp;author=D.+Wang&amp;author=D.+Y.+Yeh&amp;author=K.+Hsu&amp;author=Y.+H.+Hsu&amp;volume=126&amp;publication_year=2004&amp;pages=552-558&amp;pmid=15302744&amp;doi=10.1378\/chest.126.2.552&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B9\"><span class=\"mixed-citation\">Kawamura T., Huang C. S., Tochigi N., Lee S., Shigemura N., Billiar T. R., et al. (2010). <span class=\"ref-title\">Inhaled hydrogen gas therapy for prevention of lung transplant-induced ischemia\/reperfusion injury in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Transplantation<\/em><\/span><br \/>\n<span class=\"ref-vol\">90<\/span><br \/>\n1344\u20131351. 10.1097\/TP.0b013e3181fe1357<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/21048533\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FTP.0b013e3181fe1357\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Transplantation&amp;title=Inhaled+hydrogen+gas+therapy+for+prevention+of+lung+transplant-induced+ischemia\/reperfusion+injury+in+rats.&amp;author=T.+Kawamura&amp;author=C.+S.+Huang&amp;author=N.+Tochigi&amp;author=S.+Lee&amp;author=N.+Shigemura&amp;volume=90&amp;publication_year=2010&amp;pages=1344-1351&amp;pmid=21048533&amp;doi=10.1097\/TP.0b013e3181fe1357&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B10\"><span class=\"mixed-citation\">Kobayashi E., Sano M. (2019). <span class=\"ref-title\">Organ preservation solution containing dissolved hydrogen gas from a hydrogen-absorbing alloy canister improves function of transplanted ischemic kidneys in miniature pigs.<\/span><br \/>\n<span class=\"ref-journal\"><em>PLoS One<\/em><\/span><br \/>\n<span class=\"ref-vol\">14<\/span>:e0222863. 10.1371\/journal.pone.0222863<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6772054\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31574107\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1371%2Fjournal.pone.0222863\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=PLoS+One&amp;title=Organ+preservation+solution+containing+dissolved+hydrogen+gas+from+a+hydrogen-absorbing+alloy+canister+improves+function+of+transplanted+ischemic+kidneys+in+miniature+pigs.&amp;author=E.+Kobayashi&amp;author=M.+Sano&amp;volume=14&amp;publication_year=2019&amp;pages=e0222863&amp;pmid=31574107&amp;doi=10.1371\/journal.pone.0222863&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B11\"><span class=\"mixed-citation\">Liu R., Fang X., Meng C., Xing J., Liu J., Yang W., et al. (2015). <span class=\"ref-title\">Lung inflation with hydrogen during the cold ischemia phase decreases lung graft injury in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Exp. Biol. Med.<\/em><\/span><br \/>\n<span class=\"ref-vol\">240<\/span><br \/>\n1214\u20131222. 10.1177\/1535370214563895<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4935368\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25662956\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1177%2F1535370214563895\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Exp.+Biol.+Med.&amp;title=Lung+inflation+with+hydrogen+during+the+cold+ischemia+phase+decreases+lung+graft+injury+in+rats.&amp;author=R.+Liu&amp;author=X.+Fang&amp;author=C.+Meng&amp;author=J.+Xing&amp;author=J.+Liu&amp;volume=240&amp;publication_year=2015&amp;pages=1214-1222&amp;pmid=25662956&amp;doi=10.1177\/1535370214563895&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B12\"><span class=\"mixed-citation\">Liu X., Ma C., Wang X., Wang W., Li Z., Wang X., et al. (2017). <span class=\"ref-title\">Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model.<\/span><br \/>\n<span class=\"ref-journal\"><em>Int. J. Chron. Obstruct. Pulmon. Dis.<\/em><\/span><br \/>\n<span class=\"ref-vol\">12<\/span><br \/>\n1309\u20131324. 10.2147\/COPD.S124547<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5422326\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28496315\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.2147%2FCOPD.S124547\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Int.+J.+Chron.+Obstruct.+Pulmon.+Dis.&amp;title=Hydrogen+coadministration+slows+the+development+of+COPD-like+lung+disease+in+a+cigarette+smoke-induced+rat+model.&amp;author=X.+Liu&amp;author=C.+Ma&amp;author=X.+Wang&amp;author=W.+Wang&amp;author=Z.+Li&amp;volume=12&amp;publication_year=2017&amp;pages=1309-1324&amp;pmid=28496315&amp;doi=10.2147\/COPD.S124547&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B13\"><span class=\"mixed-citation\">Machuca T. N., Cypel M., Keshavjee S. (2013). <span class=\"ref-title\">Advances in lung preservation.<\/span><br \/>\n<span class=\"ref-journal\"><em>Surg. Clin. North Am.<\/em><\/span><br \/>\n<span class=\"ref-vol\">93<\/span><br \/>\n1373\u20131394. 10.1016\/j.suc.2013.08.001<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/24206857\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.suc.2013.08.001\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Surg.+Clin.+North+Am.&amp;title=Advances+in+lung+preservation.&amp;author=T.+N.+Machuca&amp;author=M.+Cypel&amp;author=S.+Keshavjee&amp;volume=93&amp;publication_year=2013&amp;pages=1373-1394&amp;pmid=24206857&amp;doi=10.1016\/j.suc.2013.08.001&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B14\"><span class=\"mixed-citation\">Man S. M., Karki R., Kanneganti T. D. (2017). <span class=\"ref-title\">Molecular mechanisms and functions of pyroptosis, inflammatory caspases and inflammasomes in infectious diseases.<\/span><br \/>\n<span class=\"ref-journal\"><em>Immunol. Rev.<\/em><\/span><br \/>\n<span class=\"ref-vol\">277<\/span><br \/>\n61\u201375. 10.1111\/imr.12534<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5416822\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28462526\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1111%2Fimr.12534\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Immunol.+Rev.&amp;title=Molecular+mechanisms+and+functions+of+pyroptosis,+inflammatory+caspases+and+inflammasomes+in+infectious+diseases.&amp;author=S.+M.+Man&amp;author=R.+Karki&amp;author=T.+D.+Kanneganti&amp;volume=277&amp;publication_year=2017&amp;pages=61-75&amp;pmid=28462526&amp;doi=10.1111\/imr.12534&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B15\"><span class=\"mixed-citation\">Meng C., Ma L., Niu L., Cui X., Liu J., Kang J., et al. (2016). <span class=\"ref-title\">Protection of donor lung inflation in the setting of cold ischemia against ischemia-reperfusion injury with carbon monoxide, hydrogen, or both in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Life Sci.<\/em><\/span><br \/>\n<span class=\"ref-vol\">151<\/span><br \/>\n199\u2013206. 10.1016\/j.lfs.2016.03.015<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26969763\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.lfs.2016.03.015\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Life+Sci.&amp;title=Protection+of+donor+lung+inflation+in+the+setting+of+cold+ischemia+against+ischemia-reperfusion+injury+with+carbon+monoxide,+hydrogen,+or+both+in+rats.&amp;author=C.+Meng&amp;author=L.+Ma&amp;author=L.+Niu&amp;author=X.+Cui&amp;author=J.+Liu&amp;volume=151&amp;publication_year=2016&amp;pages=199-206&amp;pmid=26969763&amp;doi=10.1016\/j.lfs.2016.03.015&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B16\"><span class=\"mixed-citation\">Ng C. S., Wan S., Arifi A. A., Yim A. P. (2006). <span class=\"ref-title\">Inflammatory response to pulmonary ischemia-reperfusion injury.<\/span><br \/>\n<span class=\"ref-journal\"><em>Surg. Today<\/em><\/span><br \/>\n<span class=\"ref-vol\">36<\/span><br \/>\n205\u2013214. 10.1007\/s00595-005-3124-2<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/16493527\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1007%2Fs00595-005-3124-2\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Surg.+Today&amp;title=Inflammatory+response+to+pulmonary+ischemia-reperfusion+injury.&amp;author=C.+S.+Ng&amp;author=S.+Wan&amp;author=A.+A.+Arifi&amp;author=A.+P.+Yim&amp;volume=36&amp;publication_year=2006&amp;pages=205-214&amp;pmid=16493527&amp;doi=10.1007\/s00595-005-3124-2&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B17\"><span class=\"mixed-citation\">Noda K., Shigemura N., Tanaka Y., Bhama J., D\u2019Cunha J., Kobayashi H., et al. (2014). <span class=\"ref-title\">Hydrogen preconditioning during ex vivo lung perfusion improves the quality of lung grafts in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Transplantation<\/em><\/span><br \/>\n<span class=\"ref-vol\">98<\/span><br \/>\n499\u2013506. 10.1097\/TP.0000000000000254<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25121557\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FTP.0000000000000254\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Transplantation&amp;title=Hydrogen+preconditioning+during+ex+vivo+lung+perfusion+improves+the+quality+of+lung+grafts+in+rats.&amp;author=K.+Noda&amp;author=N.+Shigemura&amp;author=Y.+Tanaka&amp;author=J.+Bhama&amp;author=J.+D\u2019Cunha&amp;volume=98&amp;publication_year=2014&amp;pages=499-506&amp;pmid=25121557&amp;doi=10.1097\/TP.0000000000000254&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B18\"><span class=\"mixed-citation\">Noda K., Shigemura N., Tanaka Y., Kawamura T., Hyun Lim S., Kokubo K., et al. (2013). <span class=\"ref-title\">A novel method of preserving cardiac grafts using a hydrogen-rich water bath.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Heart Lung Transplant.<\/em><\/span><br \/>\n<span class=\"ref-vol\">32<\/span><br \/>\n241\u2013250. 10.1016\/j.healun.2012.11.004<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23273745\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.healun.2012.11.004\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Heart+Lung+Transplant.&amp;title=A+novel+method+of+preserving+cardiac+grafts+using+a+hydrogen-rich+water+bath.&amp;author=K.+Noda&amp;author=N.+Shigemura&amp;author=Y.+Tanaka&amp;author=T.+Kawamura&amp;author=S.+Hyun+Lim&amp;volume=32&amp;publication_year=2013&amp;pages=241-250&amp;pmid=23273745&amp;doi=10.1016\/j.healun.2012.11.004&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B19\"><span class=\"mixed-citation\">Noda K., Tane S., Haam S. J., D\u2019Cunha J., Hayanga A. J., Luketich J. D., et al. (2017). <span class=\"ref-title\">Targeting circulating leukocytes and pyroptosis during ex vivo lung perfusion improves lung preservation.<\/span><br \/>\n<span class=\"ref-journal\"><em>Transplantation<\/em><\/span><br \/>\n<span class=\"ref-vol\">101<\/span><br \/>\n2841\u20132849. 10.1097\/TP.0000000000001798<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28452921\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FTP.0000000000001798\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Transplantation&amp;title=Targeting+circulating+leukocytes+and+pyroptosis+during+ex+vivo+lung+perfusion+improves+lung+preservation.&amp;author=K.+Noda&amp;author=S.+Tane&amp;author=S.+J.+Haam&amp;author=J.+D\u2019Cunha&amp;author=A.+J.+Hayanga&amp;volume=101&amp;publication_year=2017&amp;pages=2841-2849&amp;pmid=28452921&amp;doi=10.1097\/TP.0000000000001798&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B20\"><span class=\"mixed-citation\">Ozeki N., Yamawaki-Ogata A., Narita Y., Mii S., Ushida K., Ito M., et al. (2020). <span class=\"ref-title\">Hydrogen water alleviates obliterative airway disease in mice.<\/span><br \/>\n<span class=\"ref-journal\"><em>Gen. Thorac. Cardiovasc. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">68<\/span><br \/>\n158\u2013163. 10.1007\/s11748-019-01195-3<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31468277\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1007%2Fs11748-019-01195-3\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Gen.+Thorac.+Cardiovasc.+Surg.&amp;title=Hydrogen+water+alleviates+obliterative+airway+disease+in+mice.&amp;author=N.+Ozeki&amp;author=A.+Yamawaki-Ogata&amp;author=Y.+Narita&amp;author=S.+Mii&amp;author=K.+Ushida&amp;volume=68&amp;publication_year=2020&amp;pages=158-163&amp;pmid=31468277&amp;doi=10.1007\/s11748-019-01195-3&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B21\"><span class=\"mixed-citation\">Pirat A., Zeyneloglu P., Aldemir D., Y\u00fccel M., Ozen O., Candan S., et al. (2006). <span class=\"ref-title\">Pretreatment with simvastatin reduces lung injury related to intestinal ischemia-reperfusion in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Anesth. Analg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">102<\/span><br \/>\n225\u2013232. 10.1213\/01.ane.0000189554.41095.98 [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/16368834\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1213%2F01.ane.0000189554.41095.98\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Anesth.+Analg.&amp;title=Pretreatment+with+simvastatin+reduces+lung+injury+related+to+intestinal+ischemia-reperfusion+in+rats.&amp;author=A.+Pirat&amp;author=P.+Zeyneloglu&amp;author=D.+Aldemir&amp;author=M.+Y\u00fccel&amp;author=O.+Ozen&amp;volume=102&amp;publication_year=2006&amp;pages=225-232&amp;pmid=16368834&amp;doi=10.1213\/01.ane.0000189554.41095.98&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B22\"><span class=\"mixed-citation\">Pizanis N., Petrov A., Heckmann J., Wiswedel I., Wohlschl\u00e4ger J., de Groot H., et al. (2012). <span class=\"ref-title\">A new preservation solution for lung transplantation: evaluation in a porcine transplantation model.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Heart Lung Transplant.<\/em><\/span><br \/>\n<span class=\"ref-vol\">31<\/span><br \/>\n310\u2013317. 10.1016\/j.healun.2011.11.009<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22226803\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.healun.2011.11.009\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Heart+Lung+Transplant.&amp;title=A+new+preservation+solution+for+lung+transplantation:+evaluation+in+a+porcine+transplantation+model.&amp;author=N.+Pizanis&amp;author=A.+Petrov&amp;author=J.+Heckmann&amp;author=I.+Wiswedel&amp;author=J.+Wohlschl\u00e4ger&amp;volume=31&amp;publication_year=2012&amp;pages=310-317&amp;pmid=22226803&amp;doi=10.1016\/j.healun.2011.11.009&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B23\"><span class=\"mixed-citation\">Qiu Z., Lei S., Zhao B., Wu Y., Su W., Liu M., et al. (2017). <span class=\"ref-title\">NLRP3 Inflammasome Activation-Mediated Pyroptosis Aggravates Myocardial Ischemia\/Reperfusion Injury in Diabetic Rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Oxid. Med. Cell Longev.<\/em><\/span><br \/>\n<span class=\"ref-vol\">2017<\/span>:9743280. 10.1155\/2017\/9743280<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5618779\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29062465\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1155%2F2017%2F9743280\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Oxid.+Med.+Cell+Longev.&amp;title=NLRP3+Inflammasome+Activation-Mediated+Pyroptosis+Aggravates+Myocardial+Ischemia\/Reperfusion+Injury+in+Diabetic+Rats.&amp;author=Z.+Qiu&amp;author=S.+Lei&amp;author=B.+Zhao&amp;author=Y.+Wu&amp;author=W.+Su&amp;volume=2017&amp;publication_year=2017&amp;pages=9743280&amp;pmid=29062465&amp;doi=10.1155\/2017\/9743280&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B24\"><span class=\"mixed-citation\">Ren J. D., Ma J., Hou J., Xiao W. J., Jin W. H., Wu J., et al. (2014). <span class=\"ref-title\">Hydrogen-rich saline inhibits NLRP3 inflammasome activation and attenuates experimental acute pancreatitis in mice.<\/span><br \/>\n<span class=\"ref-journal\"><em>Mediators Inflamm.<\/em><\/span><br \/>\n<span class=\"ref-vol\">2014<\/span>:930894. 10.1155\/2014\/930894<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC4158121\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/25214720\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1155%2F2014%2F930894\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Mediators+Inflamm.&amp;title=Hydrogen-rich+saline+inhibits+NLRP3+inflammasome+activation+and+attenuates+experimental+acute+pancreatitis+in+mice.&amp;author=J.+D.+Ren&amp;author=J.+Ma&amp;author=J.+Hou&amp;author=W.+J.+Xiao&amp;author=W.+H.+Jin&amp;volume=2014&amp;publication_year=2014&amp;pages=930894&amp;pmid=25214720&amp;doi=10.1155\/2014\/930894&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B25\"><span class=\"mixed-citation\">Ren J. D., Wu X. B., Jiang R., Hao D. P., Liu Y. (2016). <span class=\"ref-title\">Molecular hydrogen inhibits lipopolysaccharide-triggered NLRP3 inflammasome activation in macrophages by targeting the mitochondrial reactive oxygen species.<\/span><br \/>\n<span class=\"ref-journal\"><em>Biochim. Biophys. Acta<\/em><\/span><br \/>\n<span class=\"ref-vol\">1863<\/span><br \/>\n50\u201355. 10.1016\/j.bbamcr.2015.10.012<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26488087\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.bbamcr.2015.10.012\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Biochim.+Biophys.+Acta&amp;title=Molecular+hydrogen+inhibits+lipopolysaccharide-triggered+NLRP3+inflammasome+activation+in+macrophages+by+targeting+the+mitochondrial+reactive+oxygen+species.&amp;author=J.+D.+Ren&amp;author=X.+B.+Wu&amp;author=R.+Jiang&amp;author=D.+P.+Hao&amp;author=Y.+Liu&amp;volume=1863&amp;publication_year=2016&amp;pages=50-55&amp;pmid=26488087&amp;doi=10.1016\/j.bbamcr.2015.10.012&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B26\"><span class=\"mixed-citation\">Saito M., Chen-Yoshikawa T. F., Takahashi M., Kayawake H., Yokoyama Y., Kurokawa R., et al. (2020). <span class=\"ref-title\">Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Thorac. Cardiovasc. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">159<\/span><br \/>\n2110\u20132118. 10.1016\/j.jtcvs.2019.09.175<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31780065\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.jtcvs.2019.09.175\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Thorac.+Cardiovasc.+Surg.&amp;title=Protective+effects+of+a+hydrogen-rich+solution+during+cold+ischemia+in+rat+lung+transplantation.&amp;author=M.+Saito&amp;author=T.+F.+Chen-Yoshikawa&amp;author=M.+Takahashi&amp;author=H.+Kayawake&amp;author=Y.+Yokoyama&amp;volume=159&amp;publication_year=2020&amp;pages=2110-2118&amp;pmid=31780065&amp;doi=10.1016\/j.jtcvs.2019.09.175&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B27\"><span class=\"mixed-citation\">Shao A., Wu H., Hong Y., Tu S., Sun X., Wu Q., et al. (2016). <span class=\"ref-title\">Hydrogen-rich saline attenuated subarachnoid hemorrhage-induced early brain injury in rats by suppressing inflammatory response: possible involvement of nf-\u03bab pathway and nlrp3 inflammasome.<\/span><br \/>\n<span class=\"ref-journal\"><em>Mol. Neurobiol.<\/em><\/span><br \/>\n<span class=\"ref-vol\">53<\/span><br \/>\n3462\u20133476. 10.1007\/s12035-015-9242-y<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/26091790\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1007%2Fs12035-015-9242-y\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Mol.+Neurobiol.&amp;title=Hydrogen-rich+saline+attenuated+subarachnoid+hemorrhage-induced+early+brain+injury+in+rats+by+suppressing+inflammatory+response:+possible+involvement+of+nf-\u03bab+pathway+and+nlrp3+inflammasome.&amp;author=A.+Shao&amp;author=H.+Wu&amp;author=Y.+Hong&amp;author=S.+Tu&amp;author=X.+Sun&amp;volume=53&amp;publication_year=2016&amp;pages=3462-3476&amp;pmid=26091790&amp;doi=10.1007\/s12035-015-9242-y&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B28\"><span class=\"mixed-citation\">Takahashi M., Chen-Yoshikawa T. F., Saito M., Tanaka S., Miyamoto E., Ohata K., et al. (2017). <span class=\"ref-title\">Immersing lungs in hydrogen-rich saline attenuates lung ischaemia-reperfusion injury.<\/span><br \/>\n<span class=\"ref-journal\"><em>Eur. J. Cardiothorac. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">51<\/span><br \/>\n442\u2013448. 10.1093\/ejcts\/ezw342<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/28364439\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1093%2Fejcts%2Fezw342\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Eur.+J.+Cardiothorac.+Surg.&amp;title=Immersing+lungs+in+hydrogen-rich+saline+attenuates+lung+ischaemia-reperfusion+injury.&amp;author=M.+Takahashi&amp;author=T.+F.+Chen-Yoshikawa&amp;author=M.+Saito&amp;author=S.+Tanaka&amp;author=E.+Miyamoto&amp;volume=51&amp;publication_year=2017&amp;pages=442-448&amp;pmid=28364439&amp;doi=10.1093\/ejcts\/ezw342&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B29\"><span class=\"mixed-citation\">Uto K., Sakamoto S., Que W., Shimata K., Hashimoto S., Sakisaka M., et al. (2019). <span class=\"ref-title\">Hydrogen-rich solution attenuates cold ischemia-reperfusion injury in rat liver transplantation.<\/span><br \/>\n<span class=\"ref-journal\"><em>BMC Gastroenterol.<\/em><\/span><br \/>\n<span class=\"ref-vol\">19<\/span>:25. 10.1186\/s12876-019-0939-7<br \/>\n <span class=\"nowrap\">[<a class=\"int-reflink\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6368804\/\">PMC free article<\/a>]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30736744\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1186%2Fs12876-019-0939-7\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=BMC+Gastroenterol.&amp;title=Hydrogen-rich+solution+attenuates+cold+ischemia-reperfusion+injury+in+rat+liver+transplantation.&amp;author=K.+Uto&amp;author=S.+Sakamoto&amp;author=W.+Que&amp;author=K.+Shimata&amp;author=S.+Hashimoto&amp;volume=19&amp;publication_year=2019&amp;pages=25&amp;pmid=30736744&amp;doi=10.1186\/s12876-019-0939-7&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B30\"><span class=\"mixed-citation\">Weder W., Harper B., Shimokawa S., Miyoshi S., Date H., Schreinemakers H., et al. (1991). <span class=\"ref-title\">Influence of intraalveolar oxygen concentration on lung preservation in a rabbit model.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Thorac. Cardiovasc. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">101<\/span><br \/>\n1037\u20131043. [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/1903825\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Thorac.+Cardiovasc.+Surg.&amp;title=Influence+of+intraalveolar+oxygen+concentration+on+lung+preservation+in+a+rabbit+model.&amp;author=W.+Weder&amp;author=B.+Harper&amp;author=S.+Shimokawa&amp;author=S.+Miyoshi&amp;author=H.+Date&amp;volume=101&amp;publication_year=1991&amp;pages=1037-1043&amp;pmid=1903825&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B31\"><span class=\"mixed-citation\">Xu K. Y., Wu C. Y., Tong S., Xiong P., Wang S. H. (2018). <span class=\"ref-title\">The selective Nlrp3 inflammasome inhibitor Mcc950 attenuates lung ischemia-reperfusion injury.<\/span><br \/>\n<span class=\"ref-journal\"><em>Biochem. Biophys. Res. Commun.<\/em><\/span><br \/>\n<span class=\"ref-vol\">503<\/span><br \/>\n3031\u20133037. 10.1016\/j.bbrc.2018.08.089<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30146255\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.bbrc.2018.08.089\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Biochem.+Biophys.+Res.+Commun.&amp;title=The+selective+Nlrp3+inflammasome+inhibitor+Mcc950+attenuates+lung+ischemia-reperfusion+injury.&amp;author=K.+Y.+Xu&amp;author=C.+Y.+Wu&amp;author=S.+Tong&amp;author=P.+Xiong&amp;author=S.+H.+Wang&amp;volume=503&amp;publication_year=2018&amp;pages=3031-3037&amp;pmid=30146255&amp;doi=10.1016\/j.bbrc.2018.08.089&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B32\"><span class=\"mixed-citation\">Zhai W., Ge J., Inci I., Hillinger S., Markus C., Korom S., et al. (2008). <span class=\"ref-title\">Simplified rat lung transplantation by using a modified cuff technique.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Invest. Surg.<\/em><\/span><br \/>\n<span class=\"ref-vol\">21<\/span><br \/>\n33\u201337. 10.1080\/08941930701834114<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/18197532\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1080%2F08941930701834114\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Invest.+Surg.&amp;title=Simplified+rat+lung+transplantation+by+using+a+modified+cuff+technique.&amp;author=W.+Zhai&amp;author=J.+Ge&amp;author=I.+Inci&amp;author=S.+Hillinger&amp;author=C.+Markus&amp;volume=21&amp;publication_year=2008&amp;pages=33-37&amp;pmid=18197532&amp;doi=10.1080\/08941930701834114&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B33\"><span class=\"mixed-citation\">Zhang G., Li Z., Meng C., Kang J., Zhang M., Ma L., et al. (2018). <span class=\"ref-title\">The Anti-inflammatory Effect of Hydrogen on Lung Transplantation Model of Pulmonary Microvascular Endothelial Cells During Cold Storage Period.<\/span><br \/>\n<span class=\"ref-journal\"><em>Transplantation<\/em><\/span><br \/>\n<span class=\"ref-vol\">102<\/span><br \/>\n1253\u20131261. 10.1097\/TP.0000000000002276<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29762455\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FTP.0000000000002276\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Transplantation&amp;title=The+Anti-inflammatory+Effect+of+Hydrogen+on+Lung+Transplantation+Model+of+Pulmonary+Microvascular+Endothelial+Cells+During+Cold+Storage+Period.&amp;author=G.+Zhang&amp;author=Z.+Li&amp;author=C.+Meng&amp;author=J.+Kang&amp;author=M.+Zhang&amp;volume=102&amp;publication_year=2018&amp;pages=1253-1261&amp;pmid=29762455&amp;doi=10.1097\/TP.0000000000002276&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B34\"><span class=\"mixed-citation\">Zhang J., Zhou H., Liu J., Meng C., Deng L., Li W. (2019). <span class=\"ref-title\">Protective effects of hydrogen inhalation during the warm ischemia phase against lung ischemia-reperfusion injury in rat donors after cardiac death.<\/span><br \/>\n<span class=\"ref-journal\"><em>Microvasc. Res<\/em>.<\/span><br \/>\n<span class=\"ref-vol\">125<\/span>:103885. 10.1016\/j.mvr.2019.103885<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31175855\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.mvr.2019.103885\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Microvasc.+Res.&amp;title=Protective+effects+of+hydrogen+inhalation+during+the+warm+ischemia+phase+against+lung+ischemia-reperfusion+injury+in+rat+donors+after+cardiac+death.&amp;author=J.+Zhang&amp;author=H.+Zhou&amp;author=J.+Liu&amp;author=C.+Meng&amp;author=L.+Deng&amp;volume=125&amp;publication_year=2019&amp;pages=103885&amp;pmid=31175855&amp;doi=10.1016\/j.mvr.2019.103885&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B35\"><span class=\"mixed-citation\">Zhou H., Fu Z., Wei Y., Liu J., Cui X., Yang W., et al. (2013). <span class=\"ref-title\">Hydrogen inhalation decreases lung graft injury in brain-dead donor rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>J. Heart Lung Transplant.<\/em><\/span><br \/>\n<span class=\"ref-vol\">32<\/span><br \/>\n251\u2013258. 10.1016\/j.healun.2012.11.007<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23273744\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1016%2Fj.healun.2012.11.007\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=J.+Heart+Lung+Transplant.&amp;title=Hydrogen+inhalation+decreases+lung+graft+injury+in+brain-dead+donor+rats.&amp;author=H.+Zhou&amp;author=Z.+Fu&amp;author=Y.+Wei&amp;author=J.+Liu&amp;author=X.+Cui&amp;volume=32&amp;publication_year=2013&amp;pages=251-258&amp;pmid=23273744&amp;doi=10.1016\/j.healun.2012.11.007&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<li id=\"B36\"><span class=\"mixed-citation\">Zou R., Wang M. H., Chen Y., Fan X., Yang B., Du J., et al. (2019). <span class=\"ref-title\">Hydrogen-rich saline attenuates acute lung injury induced by limb ischemia\/reperfusion via down-regulating chemerin and nlrp3 in rats.<\/span><br \/>\n<span class=\"ref-journal\"><em>Shock<\/em><\/span><br \/>\n<span class=\"ref-vol\">52<\/span><br \/>\n134\u2013141. 10.1097\/SHK.0000000000001194<br \/>\n [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/29847499\" ref=\"reftype=pubmed&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FSHK.0000000000001194\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Shock&amp;title=Hydrogen-rich+saline+attenuates+acute+lung+injury+induced+by+limb+ischemia\/reperfusion+via+down-regulating+chemerin+and+nlrp3+in+rats.&amp;author=R.+Zou&amp;author=M.+H.+Wang&amp;author=Y.+Chen&amp;author=X.+Fan&amp;author=B.+Yang&amp;volume=52&amp;publication_year=2019&amp;pages=134-141&amp;pmid=29847499&amp;doi=10.1097\/SHK.0000000000001194&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=8365359&amp;issue-id=374563&amp;journal-id=1464&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<div style=\"display: none; width: 202px; top: -100px; left: -100px;\" aria-live=\"assertive\" aria-hidden=\"true\" class=\"ui-helper-reset ui-ncbipopper-wrapper ui-ncbilinksmenu\">\n<ul id=\"ui-ncbiinpagenav-2\">\n<li><a href=\"#abstract-a.m.b.mtitle\">Abstract<\/a><\/li>\n<li><a href=\"#S1title\">Introduction<\/a><\/li>\n<li><a href=\"#S2title\">Materials and Methods<\/a><\/li>\n<li><a href=\"#S3title\">Results<\/a><\/li>\n<li><a href=\"#S4title\">Discussion<\/a><\/li>\n<li><a href=\"#S5title\">Data Availability Statement<\/a><\/li>\n<li><a href=\"#S6title\">Ethics Statement<\/a><\/li>\n<li><a href=\"#S7title\">Author Contributions<\/a><\/li>\n<li><a href=\"#conf1title\">Conflict of Interest<\/a><\/li>\n<li><a href=\"#h15title\">Publisher\u2019s Note<\/a><\/li>\n<li><a href=\"#fn-group-a.o.atitle\">Footnotes<\/a><\/li>\n<li><a href=\"#S9title\">Supplementary Material<\/a><\/li>\n<li><a href=\"#ref-list-a.o.ctitle\">References<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats<\/p>\n","protected":false},"author":1,"featured_media":17899,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[130],"tags":[],"disease":[844],"body-organ":[1027],"applications":[686],"test_subjects":[1517],"report-topic":[1338],"class_list":["post-26756","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hydrogen-health","disease-surgery-transplantation-2","body-organ-lung-2","applications-ventilation-2","test_subjects-rat-2","report-topic-transplantation-graft-injury-2"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v28.1 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Hydrogen Inhalation Reduces Lung Injury in Rats<\/title>\n<meta name=\"description\" content=\"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Hydrogen Inhalation Reduces Lung Injury in Rats\" \/>\n<meta property=\"og:description\" content=\"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats\" \/>\n<meta property=\"og:url\" content=\"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/\" \/>\n<meta property=\"og:site_name\" content=\"HHO Bulgaria\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/HHOBulgaria\/\" \/>\n<meta property=\"article:published_time\" content=\"2024-01-03T19:38:06+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2024-01-29T19:13:21+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1100\" \/>\n\t<meta property=\"og:image:height\" content=\"592\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/\"},\"author\":{\"name\":\"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#\\\/schema\\\/person\\\/11fc655e42cfb1690bfccd38dc15be88\"},\"headline\":\"Hydrogen Inhalation Reduces Lung Injury in Rats\",\"datePublished\":\"2024-01-03T19:38:06+00:00\",\"dateModified\":\"2024-01-29T19:13:21+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/\"},\"wordCount\":647,\"publisher\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#organization\"},\"image\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/Vodorodana-Terapia-ReportsThumb.jpg\",\"articleSection\":[\"Hydrogen Health\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/\",\"url\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/\",\"name\":\"Hydrogen Inhalation Reduces Lung Injury in Rats\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/Vodorodana-Terapia-ReportsThumb.jpg\",\"datePublished\":\"2024-01-03T19:38:06+00:00\",\"dateModified\":\"2024-01-29T19:13:21+00:00\",\"description\":\"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#primaryimage\",\"url\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/Vodorodana-Terapia-ReportsThumb.jpg\",\"contentUrl\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/Vodorodana-Terapia-ReportsThumb.jpg\",\"width\":1100,\"height\":592},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/hydrogen-inhalation-reduces-lung-injury-in-rats\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"\u041d\u0430\u0447\u0430\u043b\u043e\",\"item\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Hydrogen Inhalation Reduces Lung Injury in Rats\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#website\",\"url\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/\",\"name\":\"HHO Bulgaria\",\"description\":\"\u0413\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440 \u043d\u0430 \u0413\u0430\u0437 \u043d\u0430 \u0411\u0440\u0430\u0443\u043d \u0437\u0430 \u0434\u0438\u0437\u0435\u043b\u043e\u0432\u0438 \u0438 \u0431\u0435\u043d\u0437\u0438\u043d\u043e\u0432\u0438 \u0434\u0432\u0438\u0433\u0430\u0442\u0435\u043b\u0438 (\u0432\u043a\u043b\u044e\u0447\u0438\u0442\u0435\u043b\u043d\u043e \u0433\u0430\u0437 \u043f\u0440\u043e\u043f\u0430\u043d-\u0431\u0443\u0442\u0430\u043d \u0438 \u043c\u0435\u0442\u0430\u043d) \u00bb \u0412\u043e\u0434\u043e\u0440\u043e\u0434\u0435\u043d \u0433\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440  \u043d\u0430 \u0413\u0430\u0437 \u043d\u0430 \u0411\u0440\u0430\u0443\u043d | HHO \u0433\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440, \u043f\u0440\u043e\u0438\u0437\u0432\u0435\u0434\u0435\u043d \u0432 \u0411\u044a\u043b\u0433\u0430\u0440\u0438\u044f\",\"publisher\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#organization\",\"name\":\"HHO Bulgaria\",\"url\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2022\\\/02\\\/HHOBG-FB-Profile-2.png\",\"contentUrl\":\"https:\\\/\\\/hho-bulgaria.com\\\/wp-content\\\/uploads\\\/2022\\\/02\\\/HHOBG-FB-Profile-2.png\",\"width\":1080,\"height\":1080,\"caption\":\"HHO Bulgaria\"},\"image\":{\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/HHOBulgaria\\\/\",\"https:\\\/\\\/www.youtube.com\\\/channel\\\/UC9d5geU7TFrhO2e7MR6IelA\"]},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/hho-bulgaria.com\\\/en\\\/#\\\/schema\\\/person\\\/11fc655e42cfb1690bfccd38dc15be88\",\"name\":\"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g\",\"caption\":\"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432\"},\"sameAs\":[\"https:\\\/\\\/hho-bulgaria.com\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Hydrogen Inhalation Reduces Lung Injury in Rats","description":"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/","og_locale":"en_US","og_type":"article","og_title":"Hydrogen Inhalation Reduces Lung Injury in Rats","og_description":"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats","og_url":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/","og_site_name":"HHO Bulgaria","article_publisher":"https:\/\/www.facebook.com\/HHOBulgaria\/","article_published_time":"2024-01-03T19:38:06+00:00","article_modified_time":"2024-01-29T19:13:21+00:00","og_image":[{"width":1100,"height":592,"url":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg","type":"image\/jpeg"}],"author":"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432","twitter_card":"summary_large_image","twitter_misc":{"Written by":"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432","Est. reading time":"3 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#article","isPartOf":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/"},"author":{"name":"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432","@id":"https:\/\/hho-bulgaria.com\/en\/#\/schema\/person\/11fc655e42cfb1690bfccd38dc15be88"},"headline":"Hydrogen Inhalation Reduces Lung Injury in Rats","datePublished":"2024-01-03T19:38:06+00:00","dateModified":"2024-01-29T19:13:21+00:00","mainEntityOfPage":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/"},"wordCount":647,"publisher":{"@id":"https:\/\/hho-bulgaria.com\/en\/#organization"},"image":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#primaryimage"},"thumbnailUrl":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg","articleSection":["Hydrogen Health"],"inLanguage":"en-US"},{"@type":"WebPage","@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/","url":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/","name":"Hydrogen Inhalation Reduces Lung Injury in Rats","isPartOf":{"@id":"https:\/\/hho-bulgaria.com\/en\/#website"},"primaryImageOfPage":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#primaryimage"},"image":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#primaryimage"},"thumbnailUrl":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg","datePublished":"2024-01-03T19:38:06+00:00","dateModified":"2024-01-29T19:13:21+00:00","description":"Lung Inflation With Hydrogen During the Cold Ischemia Phase Alleviates Lung Ischemia-Reperfusion Injury by Inhibiting Pyroptosis in Rats","breadcrumb":{"@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#primaryimage","url":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg","contentUrl":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2023\/12\/Vodorodana-Terapia-ReportsThumb.jpg","width":1100,"height":592},{"@type":"BreadcrumbList","@id":"https:\/\/hho-bulgaria.com\/en\/hydrogen-inhalation-reduces-lung-injury-in-rats\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"\u041d\u0430\u0447\u0430\u043b\u043e","item":"https:\/\/hho-bulgaria.com\/en\/"},{"@type":"ListItem","position":2,"name":"Hydrogen Inhalation Reduces Lung Injury in Rats"}]},{"@type":"WebSite","@id":"https:\/\/hho-bulgaria.com\/en\/#website","url":"https:\/\/hho-bulgaria.com\/en\/","name":"HHO Bulgaria","description":"\u0413\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440 \u043d\u0430 \u0413\u0430\u0437 \u043d\u0430 \u0411\u0440\u0430\u0443\u043d \u0437\u0430 \u0434\u0438\u0437\u0435\u043b\u043e\u0432\u0438 \u0438 \u0431\u0435\u043d\u0437\u0438\u043d\u043e\u0432\u0438 \u0434\u0432\u0438\u0433\u0430\u0442\u0435\u043b\u0438 (\u0432\u043a\u043b\u044e\u0447\u0438\u0442\u0435\u043b\u043d\u043e \u0433\u0430\u0437 \u043f\u0440\u043e\u043f\u0430\u043d-\u0431\u0443\u0442\u0430\u043d \u0438 \u043c\u0435\u0442\u0430\u043d) \u00bb \u0412\u043e\u0434\u043e\u0440\u043e\u0434\u0435\u043d \u0433\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440  \u043d\u0430 \u0413\u0430\u0437 \u043d\u0430 \u0411\u0440\u0430\u0443\u043d | HHO \u0433\u0435\u043d\u0435\u0440\u0430\u0442\u043e\u0440, \u043f\u0440\u043e\u0438\u0437\u0432\u0435\u0434\u0435\u043d \u0432 \u0411\u044a\u043b\u0433\u0430\u0440\u0438\u044f","publisher":{"@id":"https:\/\/hho-bulgaria.com\/en\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/hho-bulgaria.com\/en\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/hho-bulgaria.com\/en\/#organization","name":"HHO Bulgaria","url":"https:\/\/hho-bulgaria.com\/en\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/hho-bulgaria.com\/en\/#\/schema\/logo\/image\/","url":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2022\/02\/HHOBG-FB-Profile-2.png","contentUrl":"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2022\/02\/HHOBG-FB-Profile-2.png","width":1080,"height":1080,"caption":"HHO Bulgaria"},"image":{"@id":"https:\/\/hho-bulgaria.com\/en\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/HHOBulgaria\/","https:\/\/www.youtube.com\/channel\/UC9d5geU7TFrhO2e7MR6IelA"]},{"@type":"Person","@id":"https:\/\/hho-bulgaria.com\/en\/#\/schema\/person\/11fc655e42cfb1690bfccd38dc15be88","name":"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/4995e9b05624f69b6d4e5e58936980feceb66c508ee1e767d105fa5d9ac07802?s=96&d=mm&r=g","caption":"\u0418\u0432\u0430\u0439\u043b\u043e \u041c\u043b\u0430\u0434\u0435\u043d\u043e\u0432"},"sameAs":["https:\/\/hho-bulgaria.com"]}]}},"_links":{"self":[{"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/posts\/26756","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/comments?post=26756"}],"version-history":[{"count":0,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/posts\/26756\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/media\/17899"}],"wp:attachment":[{"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/media?parent=26756"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/categories?post=26756"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/tags?post=26756"},{"taxonomy":"disease","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/disease?post=26756"},{"taxonomy":"body-organ","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/body-organ?post=26756"},{"taxonomy":"applications","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/applications?post=26756"},{"taxonomy":"test_subjects","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/test_subjects?post=26756"},{"taxonomy":"report-topic","embeddable":true,"href":"https:\/\/hho-bulgaria.com\/en\/wp-json\/wp\/v2\/report-topic?post=26756"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}