{"id":26920,"date":"2024-01-03T21:40:06","date_gmt":"2024-01-03T19:40:06","guid":{"rendered":"https:\/\/hho-bulgaria.com\/hydrogen-water-enhances-nrf2-expression-to-alleviate-lung-fibroblast-injury\/"},"modified":"2024-01-29T21:18:28","modified_gmt":"2024-01-29T19:18:28","slug":"hydrogen-water-enhances-nrf2-expression-to-alleviate-lung-fibroblast-injury","status":"publish","type":"post","link":"https:\/\/hho-bulgaria.com\/en\/hydrogen-water-enhances-nrf2-expression-to-alleviate-lung-fibroblast-injury\/","title":{"rendered":"Hydrogen water enhances Nrf2 expression to alleviate lung fibroblast injury"},"content":{"rendered":"<div class=\"jig-ncbiinpagenav\" data-jigconfig=\"smoothScroll: false, allHeadingLevels: ['h2'], headingExclude: ':hidden,.nomenu'\" id=\"ui-ncbiinpagenav-1\">\n<div class=\"fm-sec half_rhythm no_top_margin\">\n<div class=\"fm-flexbox\">\n<div class=\"fm-citation\">\n<div class=\"citation-default\">\n<div class=\"part1\"><span role=\"menubar\"><a href=\"#\" role=\"menuitem\" aria-expanded=\"false\" aria-haspopup=\"true\">Nan Fang Yi Ke Da Xue Xue Bao.<\/a><\/span> 2020 Feb 20; 40(2): 233\u2013239. <\/div>\n<div class=\"part2\"> Chinese. <span class=\"doi\"><span>doi:&nbsp;<\/span><a href=\"\/\/doi.org\/10.12122%2Fj.issn.1673-4254.2020.02.15\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=7086139&amp;issue-id=354673&amp;journal-id=3701&amp;FROM=Article%7CFront%20Matter&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">10.12122\/j.issn.1673-4254.2020.02.15<\/a><\/span><\/div>\n<\/div>\n<\/div>\n<div class=\"fm-ids\">\n<div class=\"fm-citation-pmcid\"><span class=\"fm-citation-ids-label\">PMCID: <\/span><span>PMC7086139<\/span><\/div>\n<div class=\"fm-citation-pmid\">PMID: <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32376537\">32376537<\/a><\/div>\n<\/div>\n<\/div>\n<p class=\"whole_rythm mul-switcher\">Language: <a href=\"#\" class=\"lang-sw selected\" lang=\"zh\">Chinese<\/a> | <a href=\"#\" class=\"lang-sw\" lang=\"en\">English<\/a><\/p>\n<h1 class=\"content-title\" lang=\"zh\">\u6c22\u6c14\u6c34\u901a\u8fc7\u5347\u9ad8Nrf2\u7684\u8868\u8fbe\u51cf\u8f7b\u767e\u8349\u67af\u8bf1\u5bfc\u7684\u80ba\u7ea4\u7ef4\u5316<\/h1>\n<h1 class=\"content-trans-title\" lang=\"en\">Hydrogen water alleviates paraquat-induced lung fibroblast injury <em>in vitro<\/em> by enhancing Nrf2 expression<\/h1>\n<div class=\"half_rhythm\">\n<div class=\"contrib-group fm-author\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LI%20T%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234323049280\" co-class=\"co-affbox\">\u674e \u5a77<\/a>,<sup><\/sup><a href=\"#d35e54\" rid=\"d35e54\" class=\" bio\">*<\/a> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DENG%20S%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234318010752\" co-class=\"co-affbox\">\u9093 \u6811\u8c6a<\/a>,<sup><\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LEI%20W%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234316188752\" co-class=\"co-affbox\">\u96f7 \u96ef<\/a>,<sup><\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LI%20Z%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234318636048\" co-class=\"co-affbox\">\u674e \u632f\u5764<\/a>,<sup><\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=WU%20W%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234318632944\" co-class=\"co-affbox\">\u5434 \u6587\u5a1f<\/a>,<sup><\/sup> <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ZHANG%20T%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234316758320\" co-class=\"co-affbox\">\u5f20 \u6d9b<\/a>,<sup><\/sup> and  <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DONG%20Z%5BAuthor%5D\" class=\"affpopup\" co-rid=\"_co_idm140234316755216\" co-class=\"co-affbox\">\u8463 \u662d\u5174<\/a><sup><\/sup><sup>*<\/sup><\/div>\n<div style=\"display:none\" class=\"contrib-group aff-tip\">\n<div id=\"_co_idm140234323049280\">\n<h3 class=\"no_margin\">\u674e \u5a77<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LI%20T%5BAuthor%5D\">\u674e \u5a77<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234318010752\">\n<h3 class=\"no_margin\">\u9093 \u6811\u8c6a<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DENG%20S%5BAuthor%5D\">\u9093 \u6811\u8c6a<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234316188752\">\n<h3 class=\"no_margin\">\u96f7 \u96ef<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LEI%20W%5BAuthor%5D\">\u96f7 \u96ef<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234318636048\">\n<h3 class=\"no_margin\">\u674e \u632f\u5764<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=LI%20Z%5BAuthor%5D\">\u674e \u632f\u5764<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234318632944\">\n<h3 class=\"no_margin\">\u5434 \u6587\u5a1f<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=WU%20W%5BAuthor%5D\">\u5434 \u6587\u5a1f<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234316758320\">\n<h3 class=\"no_margin\">\u5f20 \u6d9b<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ZHANG%20T%5BAuthor%5D\">\u5f20 \u6d9b<\/a><\/div>\n<\/div>\n<div id=\"_co_idm140234316755216\">\n<h3 class=\"no_margin\">\u8463 \u662d\u5174<\/h3>\n<p>\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/p>\n<div>Find articles by <a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=DONG%20Z%5BAuthor%5D\">\u8463 \u662d\u5174<\/a><\/div>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"half_rhythm\">\n<div class=\"togglers fm-copyright-license\"><a href=\"#\" class=\"pmctoggle\" rid=\"idm140234312947136_ai\">Author information<\/a> <a href=\"#\" class=\"pmctoggle\" rid=\"idm140234312947136_an\">Article notes<\/a> <a href=\"#\" class=\"pmctoggle\" rid=\"idm140234312947136_cpl\">Copyright and License information<\/a> <a href=\"\/pmc\/about\/disclaimer\/\" style=\"margin-left: 1em\">PMC Disclaimer<\/a><\/div>\n<div class=\"fm-authors-info hide half_rhythm\" id=\"idm140234312947136_ai\" style=\"display:none\">\n<div class=\"fm-affl\" id=\"aff1\">\n<sup><\/sup><br \/>\n\u6606\u660e\u533b\u79d1\u5927\u5b66\u7b2c\u4e8c\u9644\u5c5e\u533b\u9662\u547c\u5438\u4e0e\u5371\u91cd\u75c7\u79d1\u4e00\u75c5\u533a\uff0c\u4e91\u5357 \u6606\u660e 650000,<br \/>\nFirst Ward of Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Kunming Medical University, Kunming 650000, China\n<\/div>\n<div><span class=\"contrib-email\" id=\"contrib-a.h.b.e.a\">\u674e \u5a77: <a href=\"mailto:dev@null\" data-email=\"moc.qq@2440228481\" class=\"oemail\">moc.qq@2440228481<\/a&gt;; <\/span> <span class=\"contrib-email\" id=\"contrib-a.h.b.e.g\">\u8463 \u662d\u5174: <a href=\"mailto:dev@null\" data-email=\"moc.qq@1137828352\" class=\"oemail\">moc.qq@1137828352<\/a><\/span> <\/div>\n<div id=\"cor1\">\u8463\u662d\u5174\uff0c\u535a\u58eb\uff0c\u526f\u4e3b\u4efb\u533b\u5e08\uff0cE-mail: <a href=\"mailto:dev@null\" data-email=\"moc.qq@1137828352\" class=\"oemail\">moc.qq@1137828352<\/a><\/div>\n<\/div>\n<div class=\"fm-article-notes hide half_rhythm\" id=\"idm140234312947136_an\" style=\"display:none\">\n<div class=\"fm-pubdate half_rhythm\">Received 2019 Aug 25<\/div>\n<\/div>\n<div class=\"permissions half_rhythm hide\" id=\"idm140234312947136_cpl\" style=\"display:none\">\n<div class=\"fm-copyright half_rhythm\"><a href=\"\/pmc\/about\/copyright\/\">Copyright<\/a> \u7248\u6743\u6240\u6709\u00a9\u300a\u5357\u65b9\u533b\u79d1\u5927\u5b66\u5b66\u62a5\u300b\u7f16\u8f91\u90e82020<\/div>\n<div class=\"fm-copyright half_rhythm\"><a href=\"\/pmc\/about\/copyright\/\">Copyright<\/a>  \u00a92020 Journal of Southern Medical University. All rights reserved.<\/div>\n<\/div>\n<\/div>\n<div id=\"pmclinksbox\" class=\"links-box whole_rhythm hidden\" role=\"complementary\" aria-label=\"Related or updated information about this article.\"><\/div>\n<\/div>\n<div class=\"sec\"><\/div>\n<div id=\"abstract-a.h.b.o\" lang=\"zh\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><span role=\"menubar\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"menuitem\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/span><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"abstract-a.h.b.otitle\">Abstract<\/h2>\n<p><!--article-meta--><\/p>\n<div>\n<div id=\"sec-a.h.b.o.a\" class=\"sec sec-first\">\n<h3 id=\"sec-a.h.b.o.atitle\">\u76ee\u7684<\/h3>\n<p class=\"p p-first-last\">\u63a2\u8ba8\u6c22\u6c14\u6c34\u4f9d\u8d56Nrf2\u5bf9\u767e\u8349\u67af\u8bf1\u5bfc\u7684\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u589e\u6b96\uff0c\u8f6c\u5316\u53ca\u80f6\u539f\u5206\u6ccc\u7684\u5f71\u54cd\u3002<\/p>\n<\/div>\n<div id=\"sec-a.h.b.o.b\" class=\"sec\">\n<h3 id=\"sec-a.h.b.o.btitle\">\u65b9\u6cd5<\/h3>\n<p class=\"p p-first-last\">\u4eba\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL-1\uff09\u4f53\u5916\u57f9\u517b\uff0c\u91c7\u7528RNA\u5e72\u6270\u6280\u672f\u6c89\u9ed8HLC-1\u7ec6\u80de\u4e2dNrf2\u7684\u8868\u8fbe\uff0c\u8bbe\u7f6e\u5bf9\u7167\u7ec4\u3001\u767e\u8349\u67af\uff08PQ\uff09\u67d3\u6bd2\u7ec4\u3001PQ+\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u3001PQ+\u6c22\u6c14\u6c34+Nrf2siRNA\u7efc\u5408\u5e72\u9884\u7ec4\u3002\u8bbe\u7f6e\u5bf9\u7167\u7ec4\uff1a\u4eba\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL1\uff09\u6b63\u5e38\u57f9\u517b\uff1b\u67d3\u6bd2\u7ec4\uff1aHFL1+600 \u03bcmol\/L PQ 24 h\uff1b\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\uff1aHFL1+600 \u03bcmol\/L PQ24 h\u540e+\u6c22\u6c14\u6c34\uff1b\u7efc\u5408\u5e72\u9884\u6cbb\u7597\u7ec4\uff1aHFL1\u8f6c\u67d3siRNA+600 \u03bcmol\/LPQ 24 h\u540e+\u6c22\u6c14\u6c34\u3002MTT\u6cd5\u68c0\u6d4b\u7ec6\u80de\u7684\u589e\u6b96\uff0cWestern blot\u3001qt-PCR\u3001\u514d\u75ab\u8367\u5149\u68c0\u6d4b\u5404\u7ec4\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80deCol-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\u3001Nrf2\u7684\u8868\u8fbe\uff0c\u540c\u65f6ELISA\u68c0\u6d4b\u6297\u6c27\u5316\u7269SOD\u3001CAT\u3001GSH\u7684\u542b\u91cf\u3002<\/p>\n<\/div>\n<div id=\"sec-a.h.b.o.c\" class=\"sec\">\n<h3 id=\"sec-a.h.b.o.ctitle\">\u7ed3\u679c<\/h3>\n<p class=\"p p-first-last\">\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u8f83PQ\u67d3\u6bd2\u7ec4Col-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\u7684\u8868\u8fbe\u660e\u663e\u964d\u4f4e\uff0c\u5e72\u6270Nrf2\u57fa\u56e0\u8868\u8fbe\u540e\uff0c\u6c22\u6c14\u6c34\u7597\u6548\u8f83\u6cbb\u7597\u7ec4\u663e\u8457\u4e0b\u964d\uff08<em>P<\/em> &lt; 0.05\uff09\u3002\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4Nrf2\u8868\u8fbe\u91cf\u8f83PQ\u67d3\u6bd2\u7ec4\u589e\u52a0\uff08<em>P<\/em> &lt; 0.05\uff09\u3002<\/p>\n<\/div>\n<div id=\"sec-a.h.b.o.d\" class=\"sec sec-last\">\n<h3 id=\"sec-a.h.b.o.dtitle\">\u7ed3\u8bba<\/h3>\n<p class=\"p p-first-last\">\u6c22\u6c14\u6c34\u53ef\u80fd\u901a\u8fc7\u8bf1\u5bfcNrf2\u7684\u8868\u8fbe\u4fc3\u8fdb\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u589e\u6b96\u548c\u6297\u6c27\u5316\u7269\u7684\u751f\u6210\uff0c\u540c\u65f6\u6291\u5236\u5176\u8f6c\u5316\u548c\u5206\u6ccc\u529f\u80fd\u3002<\/p>\n<\/div>\n<\/div>\n<div class=\"sec\"><strong class=\"kwd-title\">Keywords: <\/strong><span class=\"kwd-text\">\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de, \u767e\u8349\u67af, \u6c22\u6c14\u6c34, \u6838\u8f6c\u5f55\u76f8\u5173\u56e0\u5b502<\/span><\/div>\n<\/div>\n<div id=\"trans-abstract-a.h.b.p\" lang=\"en\" class=\"tsec sec\">\n<h2 class=\"head no_bottom_margin\" id=\"trans-abstract-a.h.b.ptitle\">Abstract<\/h2>\n<p><!--article-meta--><\/p>\n<div>\n<div id=\"sec-a.h.b.p.a\" class=\"sec sec-first\">\n<h2 id=\"sec-a.h.b.p.atitle\">Objective<\/h2>\n<p class=\"p p-first-last\">To investigate the effects of hydrogen water on proliferation, differentiation, collagen secretion and Nrf2 expression in paraquat-induced human lung fibroblasts.<\/p>\n<\/div>\n<div id=\"sec-a.h.b.p.b\" class=\"sec\">\n<h2 id=\"sec-a.h.b.p.btitle\">Methods<\/h2>\n<p class=\"p p-first-last\"><em>In vitro<\/em> cultured human lung fibroblasts (HFL1) exposed to 600 \u03bcmol\/L paraquat (PQ) for 24 h were treated with hydrogen water with or without RNA interference of Nrf2 expression. The changes in the cell proliferation were examined using MTT assay, and the expressions of Col-I, Col-III, \u03b1-SMA and Nrf2 in the cells were detected using Western blotting, real-time quantitative PCR and immunofluorescence assay. The contents of SOD, CAT and GSH in the cells were determined with ELISA.<\/p>\n<\/div>\n<div id=\"sec-a.h.b.p.c\" class=\"sec\">\n<h2 id=\"sec-a.h.b.p.ctitle\">Results<\/h2>\n<p class=\"p p-first-last\">Compared with the PQ-exposed cells, the cells with hydrogen water treatment showed significantly lowered expressions of Col-I, Col-III, and \u03b1-SMA. Interference of Nrf2 expression obviously attenuated the effect of hydrogen water on PQ-exposed cells. Hydrogen water treatment significantly increased the expression of Nrf2 and promoted the production of the antioxidants in PQ-exposed lung fibroblasts.<\/p>\n<\/div>\n<div id=\"sec-a.h.b.p.d\" class=\"sec sec-last\">\n<h2 id=\"sec-a.h.b.p.dtitle\">Conclusion<\/h2>\n<p class=\"p p-first-last\">Hydrogen water enhances Nrf2 expression to promote the proliferation and production of antioxidants and inhibit the differentiation and collagen secretion in PQ-exposed human lung fibroblasts <em>in vitro.<\/em><\/p>\n<\/div>\n<\/div>\n<div class=\"sec\"><strong class=\"kwd-title\">Keywords: <\/strong><span class=\"kwd-text\">lung fibroblasts, paraquat, hydrogen water, Nrf2<\/span><\/div>\n<\/div>\n<div id=\"body-a.i\" class=\"tsec sec\">\n<h2 class=\"headless nomenu\"><\/h2>\n<p class=\"p p-first\">\u80ba\u7ea4\u7ef4\u5316\u662f\u4e00\u79cd\u7531\u5404\u79cd\u56e0\u7d20\u5f15\u8d77\u80ba\u6ce1\u6301\u7eed\u6027\u635f\u4f24\u3001\u7ec6\u80de\u5916\u57fa\u8d28\u8fc7\u5ea6\u6c89\u79ef\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\u5bf9\u80ba\u90e8\u53d7\u635f\u8c03\u8282\u4e0d\u53d7\u63a7\u5236\uff0c\u4ece\u800c\u5bfc\u81f4\u80ba\u90e8\u7ea4\u7ef4\u5316\u3001\u6b63\u5e38\u80ba\u7ec4\u7ec7\u7ed3\u6784\u6539\u53d8\u4ee5\u53ca\u529f\u80fd\u4e27\u5931\u7684\u75be\u75c5<sup>[<a href=\"#b1\" rid=\"b1\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">1<\/a>&#8211;<a href=\"#b3\" rid=\"b3\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">3<\/a>]<\/sup>\u3002\u5c3d\u7ba1\u8fd1\u5e74\u6765\u6211\u4eec\u5bf9IPF\u53d1\u75c5\u673a\u7406\u7684\u8ba4\u8bc6\u548c\u6cbb\u7597\u6709\u4e86\u4e00\u5b9a\u7684\u8fdb\u5c55\uff0c\u4f46IPF\u60a3\u8005\u7684\u9884\u540e\u72b6\u51b5\u4f9d\u7136\u4e0d\u5bb9\u4e50\u89c2\u3002\u56e0\u6b64\uff0c\u4e3a\u80ba\u7ea4\u7ef4\u5316\u7684\u6cbb\u7597\u5bfb\u627e\u65b0\u7684\u5207\u5165\u9776\u70b9\u5341\u5206\u91cd\u8981\u3002\u6c22\u6c14\u4f5c\u4e3a\u6cbb\u7597\u6027\u7684\u533b\u7528\u6c14\u4f53\uff0c\u5df2\u7ecf\u5728\u591a\u79cd\u75be\u75c5\u7684\u5b9e\u9a8c\u548c\u4e34\u5e8a\u4e2d\u88ab\u52a0\u4ee5\u8bc1\u5b9e\uff0c\u4f46\u5230\u76ee\u524d\u4e3a\u6b62\uff0c\u5173\u4e8e\u6c22\u6c14\u7684\u5177\u4f53\u6548\u5e94\u673a\u5236\u5e76\u4e0d\u6e05\u695a<sup>[<a href=\"#b4\" rid=\"b4\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">4<\/a>&#8211;<a href=\"#b5\" rid=\"b5\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">5<\/a>]<\/sup>\u3002\u6709\u7814\u7a76\u89c2\u5bdf\u4e86\u6c22\u6c14\u6c34\u51cf\u8f7b\u4e86\u767e\u8349\u67af\uff08PQ\uff09\u8bf1\u5bfc\u7684\u6025\u6027\u80ba\u635f\u4f24\uff0c\u4f46\u5bf9\u5176\u5177\u4f53\u7684\u673a\u5236\u5e76\u6ca1\u6709\u8be6\u7ec6\u7684\u89e3\u91ca<sup>[<a href=\"#b5\" rid=\"b5\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">5<\/a>]<\/sup>\u3002PQ\u4e2d\u6bd2\u662f\u4e00\u4e2a\u4e16\u754c\u6027\u96be\u9898, \u4e2d\u6bd2\u540e\u7684\u6b7b\u4ea1\u7387\u9ad8\u8fbe50%~80%\uff0c\u8fc4\u4eca\u4e3a\u6b62\u5c1a\u65e0\u6709\u6548\u7684\u89e3\u6bd2\u836f<sup>[<a href=\"#b6\" rid=\"b6\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">6<\/a>]<\/sup>\u3002\u7814\u7a76\u53d1\u73b0\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u5728PQ\u4e2d\u6bd2\u4e2d\u5177\u6709\u975e\u5e38\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u800cNrf2\u4f5c\u4e3a\u6297\u6c27\u5316\u635f\u4f24\u7684\u91cd\u8981\u8c03\u8282\u56e0\u5b50\uff0c\u5728\u63d0\u9ad8\u7ec6\u80de\u6297\u6c27\u5316\u3001\u6297\u708e\u3001\u6297\u51cb\u4ea1\u7b49\u65b9\u9762\u6709\u7740\u91cd\u8981\u4f5c\u7528<sup>[<a href=\"#b7\" rid=\"b7\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">7<\/a>]<\/sup>\u3002\u7814\u7a76\u53d1\u73b0\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u5728PQ\u4e2d\u6bd2\u4e2d\u5177\u6709\u975e\u5e38\u91cd\u8981\u7684\u4f5c\u7528<sup>[<a href=\"#b8\" rid=\"b8\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">8<\/a>]<\/sup>\uff0c\u800cNrf2\u4f5c\u4e3a\u6297\u6c27\u5316\u635f\u4f24\u7684\u91cd\u8981\u8c03\u8282\u56e0\u5b50\uff0c\u5728\u63d0\u9ad8\u7ec6\u80de\u6297\u6c27\u5316\u3001\u6297\u708e\u3001\u6297\u51cb\u4ea1\u7b49\u65b9\u9762\u6709\u7740\u91cd\u8981\u4f5c\u7528<sup>[<a href=\"#b9\" rid=\"b9\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">9<\/a>&#8211;<a href=\"#b10\" rid=\"b10\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">10<\/a>]<\/sup>\uff0c\u4f46\u5728\u80ba\u7ea4\u7ef4\u5316\u4e2d\u7684\u5177\u4f53\u673a\u5236\u5c1a\u672a\u9610\u660e\u3002\u6c22\u6c14\u4f5c\u4e3a\u6297\u6c27\u5316\u5e94\u6fc0\u6cbb\u7597\u7269\u8d28\uff0c\u662f\u5426\u901a\u8fc7\u8c03\u8282Nrf2\u8868\u8fbe\u5728\u80ba\u7ea4\u7ef4\u5316\u4e2d\u53d1\u6325\u4f5c\u7528\u5c1a\u4e0d\u6e05\u695a\u3002\u672c\u5b9e\u9a8c\u65e8\u5728\u901a\u8fc7\u5e72\u6270Nrf2\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u89c2\u5bdf\u6c22\u6c14\u6c34\u6cbb\u7597PQ\u8bf1\u5bfc\u7684\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\u7684\u7597\u6548\uff0c\u4ee5\u63a2\u8ba8\u6c22\u6c14\u6c34\u6297\u6c27\u5316\u53cd\u5e94\u5728\u80ba\u7ea4\u7ef4\u5316\u4e2d\u53d1\u6325\u4f5c\u7528\u7684\u53ef\u80fd\u673a\u5236\uff0c\u4e3a\u80ba\u7ea4\u7ef4\u5316\u7684\u6cbb\u7597\u63d0\u4f9b\u65b0\u7684\u65b9\u5411\u3002<\/p>\n<\/div>\n<div id=\"s1\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"s1title\">1.\u2003\u6750\u6599\u548c\u65b9\u6cd5<\/h2>\n<div id=\"s1-1\" class=\"sec sec-first\">\n<h3 id=\"s1-1title\">1.1. \u6750\u6599<\/h3>\n<p class=\"p p-first-last\">\u4eba\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL-1\uff09\uff0c\u6606\u660e\u52a8\u7269\u7814\u7a76\u6240\u63d0\u4f9b\uff1b\u767e\u8349\u67af(\u6c5f\u82cf\u5357\u901a\u6b63\u8fbe\u4fdd\u62a4\u6709\u9650\u516c\u53f8\uff09\uff1bDMEM\u57f9\u517b\u57fa\u3001\u80ce\u725b\u8840\u6e05\u30010.25%\u80f0\u9176\uff08Gibco\uff09\uff1b\u9752\u9709\u7d20\u3001\u94fe\u9709\u7d20\uff08\u78a7\u4e91\u5929\uff09\uff1b\u603bRNA\u63d0\u53d6\u8bd5\u5242\u76d2\u3001\u9006\u8f6c\u5f55\u8bd5\u5242\u76d2\u3001\u86cb\u767d\u63d0\u53d6\u8bd5\u5242\u76d2\u3001\u5185\u53c2\u6297\u4f53\u03b2-actin\u3001\u9f20\/\u5154\u4e8c\u6297\u53ca\u8367\u5149\u4e8c\u6297\uff08\u4e07\u7c7b\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\uff09\uff1b\u4e00\u6297Nrf2\u3001Col-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\uff08Abcam\uff09\uff1bELISA\u68c0\u6d4bSOD\u3001CAT\u548cGSH\u8bd5\u5242\u76d2\uff08\u5357\u4eac\u5efa\u6210\u751f\u7269\u5de5\u7a0b\u7814\u7a76\u6240\uff09\uff1bPCR\u5f15\u7269\uff08\u4e0a\u6d77\u751f\u7269\u5de5\u7a0b\u6280\u672f\u6709\u9650\u516c\u53f8\uff09\uff1bsiRNA\u5f15\u7269\uff08\u5409\u739b\u5236\u836f\u6709\u9650\u516c\u53f8\uff09\uff0cNrf2siRNA\u6b63\u4e49\u94fe\uff1a5&#8242;-GUAAGAAGCCAGAUGUUAAdUdU-3&#8217;\uff0c\u53cd\u4e49\u94fe\uff1a5&#8242;-dUdUCAUUCUUCGGUCUACAATT-3&#8217;\u3002<\/p>\n<\/div>\n<div id=\"s1-2\" class=\"sec sec-last\">\n<h3 id=\"s1-2title\">1.2. \u65b9\u6cd5<\/h3>\n<div id=\"s1-2-1\" class=\"sec sec-first\">\n<h4 id=\"s1-2-1title\" class=\"inline\">1.2.1.  HFL-1\u7ec6\u80de\u7684\u57f9\u517b\u53ca\u9274\u5b9a <\/h4>\n<p class=\"p p-first-last\">HFL-1\u7ec6\u80de\u5728\u542b10%\u80ce\u725b\u8840\u6e05\u7684DMEM\u57f9\u517b\u57fa\u4e2d\uff0c37 \u2103\uff0c5% CO<sub>2<\/sub>\u6761\u4ef6\u4e0b\u57f9\u517b\uff0c\u53d6\u5bf9\u6570\u751f\u957f\u671f\u7684\u7ec6\u80de, \u4f7f\u75280.25%\u80f0\u9176\u6d88\u5316\u6cd51:2\u8fdb\u884c\u4f20\u4ee3\u3002<\/p>\n<\/div>\n<div id=\"s1-2-2\" class=\"sec\">\n<h4 id=\"s1-2-2title\" class=\"inline\">1.2.2.  siRNA\u6c89\u9ed8HFL-1\u7ec6\u80de\u4e2dNrf2\u7684\u8868\u8fbe <\/h4>\n<p class=\"p p-first-last\">\u8bbe\u8ba1\u5e76\u5408\u62103\u6761\u9488\u5bf9Nrf2\u57fa\u56e0\u7684siRNA\u5e8f\u5217\uff0c\u901a\u8fc7Western blot\uff0cqRT-PCR\u68c0\u6d4bRNAi\u6c89\u9ed8\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e2d\u7684Nrf2\u7684\u8868\u8fbe\uff0c\u7b5b\u9009\u6c89\u9ed8\u6548\u679c\u6700\u597d\u7684RNA\u7247\u6bb5\uff0c\u6784\u5efa\u8d28\u7c92\u8868\u8fbe\u8f7d\u4f53\uff0cNrf2-siRNA\u8868\u8fbe\u8d28\u7c92\u8f6c\u67d3\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u3002<\/p>\n<\/div>\n<div id=\"s1-2-3\" class=\"sec\">\n<h4 id=\"s1-2-3title\" class=\"inline\">1.2.3.  \u5b9e\u9a8c\u5206\u7ec4\u53ca\u6837\u672c\u63d0\u53d6 <\/h4>\n<p class=\"p p-first-last\">\u53d6\u7b2c3\u4ee3\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff0c\u968f\u673a\u5206\u4e3aD1\u7ec4\uff1a\u4eba\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL1\uff09\u6b63\u5e38\u57f9\u517b\uff1bD2\u7ec4\uff1aHFL1+600 \u03bcmol\/L PQ 24 h\uff1bD3\u7ec4\uff1aHFL1+600 mol\/L PQ 24 h\u540e+\u6c22\u6c14\u6c34\uff1bD4\u7ec4\uff1aHFL1\u8f6c\u67d3siRNA+600 mol\/L PQ 24 h\u540e+\u6c22\u6c14\u6c34\u3002\u6c22\u6c14\u6c34\u63092\u00d710<sup>5<\/sup>\u4e2a\/mL\u52a0800 \u03bcL\u7528\u91cf\u5e72\u988448 h\u540e\uff0c\u63d0\u53d6\u5404\u7ec4\u86cb\u767d\u53caRNA\u7528\u4e8e\u540e\u7eed\u5b9e\u9a8c\u3002<\/p>\n<\/div>\n<div id=\"s1-2-4\" class=\"sec\">\n<h4 id=\"s1-2-4title\" class=\"inline\">1.2.4.  MTT\u6cd5\u68c0\u6d4b\u7ec6\u80de\u589e\u6b96 <\/h4>\n<p class=\"p p-first-last\">\u53d6\u751f\u957f\u826f\u597d\u7ec6\u80de4\u00d710<sup>3<\/sup>\/\u5b54\u63a5\u79cd\u4e8e96\u5b54\u677f\uff0c37 \u2103\uff0c5% CO<sub>2<\/sub>\u6761\u4ef6\u4e0b\u57f9\u517b24 h\uff0c\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\uff0c\u8bbe\u7f6e\u8c03\u96f6\u5b54\u3001\u5bf9\u7167\u5b54\u3001\u5206\u7ec4\u6837\u672c\u5b54\u6bcf\u7ec43\u4e2a\u590d\u5b54\u3002\u6309\u5206\u7ec4\u5904\u7406\u7ec6\u80de\u540e\u5b75\u80b224 h\uff0c\u6bcf\u5b54\u52a0\u516510 \u03bcL MTT\uff085 mg\/mL\uff09\u5de5\u4f5c\u6db2\uff0c\u6c34\u5e73\u6447\u5e8a\u4f4e\u901f\u6df7\u53001 min\u540e\uff0c37 \u2103\uff0c5% CO<sub>2<\/sub>\u57f9\u517b\u7bb1\u4e2d\u5b75\u80b24 h\u3002\u5438\u5c3d\u5b54\u5185\u57f9\u517b\u6db2\uff0c\u6bcf\u5b54\u52a0\u5165100 \u03bcL\u7532\u6512\u6eb6\u89e3\u6db2\uff0c\u6df7\u5300\u540e\u57f9\u517b\u7bb1\u4e2d\u5b75\u80b24 h\uff0c\u9176\u6807\u4eea\u68c0\u6d4b\u5404\u5b54\u5728\u6ce2\u957f570 nm\u7684\u5438\u5149\u503c\u3002\u5206\u522b\u4e8e0\u30014\u30018\u300112\u300116\u300120\u300124 h\u68c0\u6d4b\u7ec6\u80de\u589e\u6b96\u72b6\u51b5\u3002<\/p>\n<\/div>\n<div id=\"s1-2-5\" class=\"sec\">\n<h4 id=\"s1-2-5title\" class=\"inline\">1.2.5.  Western blot\u68c0\u6d4bCol-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\u3001Nrf2\u86cb\u767d\u7684\u8868\u8fbe <\/h4>\n<p class=\"p p-first-last\">\u6839\u636e\u5404\u6837\u672c\u8d28\u91cf\u53ca\u4f53\u79ef\u52a0\u5165\u76f8\u5e94\u7684\u9884\u51b7\u7ec6\u80de\u88c2\u89e3\u6db2\uff0c\u7f6e\u51b0\u4e0a5 min\u5145\u5206\u88c2\u89e3\uff0c12 000 r\/min\uff0c4 \u2103\u4f4e\u6e29\u51b7\u51bb\u79bb\u5fc3\u673a\u79bb\u5fc310 min\uff0c\u53d6\u4e0a\u6e05\u3002BSA\u6cd5\u6d4b\u5b9a\u86cb\u767d\u6d53\u5ea6\uff0c\u6839\u636e\u6d4b\u5b9a\u6d53\u5ea6\u4ee550 \u03bcg\u6bcf\u5b54\u8ba1\u7b97\u4e0a\u6837\u4f53\u79ef\u3002SDS-PAGE\u7535\u6cf3\u540e\u8f6c\u819c\uff0c5%\u8131\u8102\u5976\u7c89\u5c01\u95ed1 h\uff0c\u5b75\u80b2\u4e00\u6297\uff0c\u5185\u53c2\u6297\u4f534 \u2103\u8fc7\u591c\uff0c\u6d17\u819c\u540e\u5b75\u80b2\u4e8c\u62971 h\uff0c\u53d6\u819cECL\u53d1\u5149\u6db2\u663e\u5f71\u3002ImageJ\u8f6f\u4ef6\u4ee5\u03b2-actin\u5185\u53c2\u6bd4\u503c\u4f5c\u4e3a\u534a\u5b9a\u91cf\u4f9d\u636e\uff0c\u6d4b\u5b9aWb\u56fe\u50cf\u7070\u5ea6\u503c\u3002<\/p>\n<\/div>\n<div id=\"s1-2-6\" class=\"sec\">\n<h4 id=\"s1-2-6title\" class=\"inline\">1.2.6.  Rt-PCR\u68c0\u6d4bCol-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\u3001Nrf2mRNA\u7684\u8868\u8fbe <\/h4>\n<p class=\"p p-first-last\">RNA\u63d0\u53d6\u8bd5\u5242\u76d2\u63d0\u53d6\u603bRNA\uff0c\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u68c0\u6d4bRNA\u8d28\u91cf\uff0c\u9006\u8f6c\u5f55\u8bd5\u5242\u76d2\u5236\u5907\u6269\u589e\u6a21\u677f\uff0c\u6837\u672c\u63a5\u79cd\u4e8e96\u5b54\u677f\uff0c\u6bcf\u7ec43\u4e2a\u590d\u5b54\u5e76\u8bbe\u7f6e\u9634\u6027\u5bf9\u7167\u3002\u53cd\u5e94\u7a0b\u5e8f\u4e3a95 \u2103\u53d8\u602710 min\uff0c95 \u2103\u53d8\u602730 s\u300158 \u2103\u9000\u706b30 s\u300172 \u2103 30 min\uff0c40\u4e2a\u5faa\u73af\uff0c72 \u2103\u5ef6\u4f3810 min\u3002\u68c0\u6d4b\u5404\u6a21\u677fCT\u503c\u3002CT\u503c\u4e3a\u6bcf\u4e2a\u53cd\u5e94\u7ba1\u5185\u7684\u8367\u5149\u4fe1\u53f7\u8fbe\u5230\u8bbe\u5b9a\u9608\u503c\u65f6\u6240\u7ecf\u5386\u7684\u5faa\u73af\u6570\u76ee\u3002\u25b3\u25b3CT=\u25b3CT\uff08\u5b9e\u9a8c\u503c\uff09-\u25b3CT\uff08\u7a7a\u767d\u503c\uff09\u3002\u4ee5Folds=2<sup>-\u25b3 \u25b3<\/sup><br \/>\n<sup>CT<\/sup>\u516c\u5f0f\u8ba1\u7b97\u6837\u672c\u4e2dmRNA\u7684\u8868\u8fbe\u91cf\u3002\u6240\u6709\u5b9e\u9a8c\u91cd\u590d3\u6b21\uff0c\u8ba1\u7b97\u5e73\u5747\u503c\u4e3a\u5b9e\u9a8c\u7ed3\u679c\u3002\u5f15\u7269\u5982\u4e0b\uff1aNrf2\uff08\u6b63\u4e49\u94fe\uff1a5&#8242;-TCTCCATATCC CATTCCC-3&#8217;\uff0c\u53cd\u4e49\u94fe\uff1a5&#8242;-AAGGTGCTGAGTTGTTT T-3&#8217;\uff09\uff1bCol-\u2160\uff08\u6b63\u4e49\u94fe\uff1a5&#8242;-GCAAGAACAGCGTAGCC TACAT-3&#8217;\uff0c\u53cd\u4e49\u94fe\uff1a5&#8242;-GCAGCCATCCACAAGCGT-3&#8217;\uff09\uff1bCol-\u2162\uff08\u6b63\u4e49\u94fe\uff1a5&#8242;-AGATGATGGTGCTGAGAAG-3&#8217;\uff0c\u53cd\u4e49\u94fe\uff1a5&#8242;-TGGAAAGAAGTCTGAGGAAGG-3&#8217;\uff09\uff1b\u03b1- SMA\uff08\u6b63\u4e49\u94fe\uff1a5&#8242;-AGGTAACGAGTCAGAGCTTTGG C-3&#8217;\uff0c\u53cd\u4e49\u94fe\uff1a5&#8242;-CTCTCTGTCCACCTTCCAGCAG-3&#8217;\uff09\u3002<\/p>\n<\/div>\n<div id=\"s1-2-7\" class=\"sec\">\n<h4 id=\"s1-2-7title\" class=\"inline\">1.2.7.  \u514d\u75ab\u8367\u5149\u68c0\u6d4bCol-\u2160\u3001Col-\u2162\u3001\u03b1-SMA\u3001Nrf2\u7684\u8868\u8fbe <\/h4>\n<p class=\"p p-first-last\">\u751f\u957f\u826f\u597d\u7ec6\u80de3\u00d710<sup>5<\/sup> \/\u5b54\u63a5\u79cd\u4e8e\u94fa\u597d\u722c\u7247\u768424\u5b54\u677f\uff0c37 \u2103\uff0c5% CO<sub>2<\/sub>\u6761\u4ef6\u4e0b\u57f9\u517b24 h\uff0c\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\u540e\u6309\u4e0d\u540c\u5206\u7ec4\u5904\u7406\u7ec6\u80de\uff0c\u6bcf\u7ec43\u4e2a\u590d\u5b54\uff0c\u8bbe\u7f6e\u5bf9\u7167\u5b54\u3001\u8c03\u96f6\u5b54\uff0c24 h\u540e\u8fdb\u884c\u514d\u75ab\u8367\u5149\u68c0\u6d4b\u3002\u5438\u5c3d\u57f9\u517b\u57fa\u540ePBS\u6d78\u6d173\u6b21\uff0c\u75284%\u591a\u805a\u7532\u919b\u56fa\u5b9a\u722c\u724730 min\uff0cPBS\u6d78\u6d173\u6b21\u540e\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05\u5ba4\u6e29\u5c01\u95ed30 min\uff0c\u53bb\u9664\u5c01\u95ed\u6db2\u52a0\u4e00\u6297\u6e7f\u76d2\u5b75\u80b2\u8fc7\u591c\uff0cPBST\u6d78\u6d173\u6b21\u540e\u8367\u5149\u4e8c\u6297\u907f\u5149\u5b75\u80b21 h\uff0c\u6d78\u6d17\u540eDAPI\u67d3\u683810 min\uff0cPBST\u6d78\u6d17\u540e\u5c01\u7247\u770b\u7247\uff0c\u5206\u6790\u7ed3\u679c\u3002<\/p>\n<\/div>\n<div id=\"s1-2-8\" class=\"sec\">\n<h4 id=\"s1-2-8title\" class=\"inline\">1.2.8.  ELISA\u68c0\u6d4b\u6297\u6c27\u5316\u7269SOD\u3001CAT\u3001GSH\u86cb\u767d\u7684\u542b\u91cf <\/h4>\n<p class=\"p p-first-last\">\u53d6\u751f\u957f\u826f\u597d\u7ec6\u80de\u86cb\u767d\u88c2\u89e3\u6db2\u63d0\u53d6\u86cb\u767d\uff0c\u6309SOD\u3001CAT\u3001GSH ELISA\u68c0\u6d4b\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u64cd\u4f5c\u3002<\/p>\n<\/div>\n<div id=\"s1-2-9\" class=\"sec sec-last\">\n<h4 id=\"s1-2-9title\" class=\"inline\">1.2.9.  \u7edf\u8ba1\u5b66\u5206\u6790 <\/h4>\n<p class=\"p p-first-last\">\u672c\u5b9e\u9a8c\u6570\u636e\u91c7\u7528\u5747\u6570\u00b1\u6807\u51c6\u5dee\u8868\u793a\uff0c\u4f7f\u7528SPSS18.0\u7edf\u8ba1\u5b66\u8f6f\u4ef6\u8fdb\u884c\u5355\u56e0\u7d20\u65b9\u5dee\u5206\u6790\u3002\u4f7f\u7528<em>t<\/em>\u68c0\u9a8c\u8fdb\u884c\u7ec4\u95f4\u7edf\u8ba1\u5b66\u6bd4\u8f83\uff0c<em>P<\/em> &lt; 0.05\u8ba4\u4e3a\u5dee\u5f02\u5177\u6709\u7edf\u8ba1\u5b66\u610f\u4e49\u3002<\/p>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"s2title\">2.\u2003\u7ed3\u679c<\/h2>\n<div id=\"s2-1\" class=\"sec sec-first\">\n<h3 id=\"s2-1title\">2.1. Nrf2-siRNA\u6c89\u9ed8HFL-1\u7ec6\u80de\u4e2dNrf2\u7684\u8868\u8fbe<\/h3>\n<p class=\"p p-first\">\u8bbe\u8ba1\u5e76\u5408\u62103\u6761\u9488\u5bf9Nrf2\u57fa\u56e0\u7684siRNA\u5e8f\u5217\uff0c\u8bbe\u7f6e\u7a7a\u767d\u5bf9\u7167\u7ec4\u3001siRNA\u5bf9\u7167\u7ec4\u3001siRNA- Nrf2\uff08siRNA-Nrf2-1\uff0csiRNA-Nrf2-2\uff0csiRNA-Nrf2-3\uff09\u7ec4\uff0cRt-PCR\u68c0\u6d4b\u5404\u7ec4Nrf2\u7ed3\u679c\u663e\u793a\uff0cNrf2\u8868\u8fbe\u91cf\u7a7a\u767d\u5bf9\u7167\u7ec4\u6700\u9ad8\uff0csiRNA\u5bf9\u7167\u7ec4\u4e0e\u7a7a\u767d\u5bf9\u7167\u7ec4\u8868\u8fbe\u91cf\u4e00\u81f4\uff0csiRNA-Nrf2\u7ec4\u4e0e\u7a7a\u767d\u5bf9\u7167\u7ec4\u6bd4\u8f83\u5747\u964d\u4f4e\uff0c\u5176\u4e2dsiRNA-Nrf2-3 Nrf2\u8868\u8fbe\u91cf\u6700\u4f4e\uff0c\u6291\u5236\u6548\u679c\u6700\u660e\u663e\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure1\" rid-ob=\"ob-Figure1\" co-legend-rid=\"lgnd_Figure1\" rel=\"noopener\"><span>\u56fe 1A<\/span><\/a>\uff09\u3002\u7b5b\u9009siRNA-Nrf2-3\u6784\u5efa\u8d28\u7c92\u8868\u8fbe\u8f7d\u4f53\u8f6c\u67d3\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7528\u4e8e\u540e\u7eed\u5206\u7ec4\u5904\u7406\u3002Western blot\u68c0\u6d4b\u5404\u7ec4Nrf2\u7ed3\u679c\u663e\u793a\uff0cNrf2\u8868\u8fbe\u91cf\u7a7a\u767d\u5bf9\u7167\u7ec4\u6700\u9ad8\uff0csiRNA\u5bf9\u7167\u7ec4\u4e0e\u7a7a\u767d\u5bf9\u7167\u7ec4\u8868\u8fbe\u91cf\u4e00\u81f4\uff0csiRNA-Nrf2-3\u7ec4\u8868\u8fbe\u91cf\u88ab\u660e\u663e\u6291\u5236\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure1\" rid-ob=\"ob-Figure1\" co-legend-rid=\"lgnd_Figure1\" rel=\"noopener\"><span>\u56fe 1B<\/span><\/a>\uff09\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure1\" co-legend-rid=\"lgnd_Figure1\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/\" target=\"figure\" rid-figpopup=\"Figure1\" rid-ob=\"ob-Figure1\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234322408336\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-1.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-1.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-1.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234322408336\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/\" target=\"figure\" rid-figpopup=\"Figure1\" rid-ob=\"ob-Figure1\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure1\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure1\/\" target=\"figure\" rid-figpopup=\"Figure1\" rid-ob=\"ob-Figure1\" rel=\"noopener\">1<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4Nrf2\u7684\u8868\u8fbe<\/p>\n<p>Nrf2 expression in blank control group, siRNA control group, siRNA- Nrf2-1 group, siRNA- Nrf2- 2 group and siRNA- Nrf2-3 group. <sup>**<\/sup><em>P<\/em> &lt; 0.01 <em>vs<\/em> HFL-1 group; <sup>***<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> HFL-1 group; <sup>++<\/sup><br \/>\n<em>P<\/em> &lt; 0.01 <em>vs<\/em> siRNA NC group; <sup>+++<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> siRNA NC group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2-2\" class=\"sec\">\n<h3 id=\"s2-2title\">2.2. \u6c22\u6c14\u6c34\u589e\u5f3aPQ\u67d3\u6bd2\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80deNrf2\u7684\u8868\u8fbe<\/h3>\n<p class=\"p p-first\">Western blot\u68c0\u6d4b\u5404\u7ec4Nrf2\u7ed3\u679c\u663e\u793a\uff0c\u7a7a\u767d\u5bf9\u7167\u7ec4Nrf2\u86cb\u767d\u8868\u8fbe\u91cf\u6700\u4f4e\uff0cPQ\u67d3\u6bd2\u7ec4\u660e\u663e\u589e\u52a0\uff0c\u800cPQ 24 h+\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u8868\u8fbe\u91cf\u6700\u9ad8\uff0cPQ 24 h+\u6c22\u6c14\u6c34+siRNA\u7efc\u5408\u5e72\u9884\u7ec4\u8868\u8fbe\u91cf\u9ad8\u4e8e\u7a7a\u767d\u5bf9\u7167\u7ec4\uff0c\u4f46\u4ecb\u4e8e\u7a7a\u767d\u5bf9\u7167\u7ec4\u548c\u67d3\u6bd2\u7ec4\u4e4b\u95f4\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" co-legend-rid=\"lgnd_Figure2\" rel=\"noopener\"><span>\u56fe 2A<\/span><\/a>\uff09\u3002Rt-PCR\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" co-legend-rid=\"lgnd_Figure2\" rel=\"noopener\"><span>\u56fe 2B<\/span><\/a>\uff09\uff0c\u514d\u75ab\u8367\u5149\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" co-legend-rid=\"lgnd_Figure2\" rel=\"noopener\"><span>\u56fe 2C<\/span><\/a>\uff09\u7ed3\u679c\u4e0eWestern blot\u7ed3\u679c\u4e00\u81f4\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure2\" co-legend-rid=\"lgnd_Figure2\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234357478704\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-2.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-2.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-2.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234357478704\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure2\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure2\/\" target=\"figure\" rid-figpopup=\"Figure2\" rid-ob=\"ob-Figure2\" rel=\"noopener\">2<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80deNrf2\u7684\u8868\u8fbe<\/p>\n<p>Expression of Nrf2 in lung fibroblasts in control group (D1), PQ poisoning group (D2), PQ + hydrogen water treatment group (D3), and PQ + hydrogen water + siRNA group (D4) (Original magnification: \u00d740). <sup>*<\/sup><em>P<\/em> &lt; 0.05 <em>vs<\/em> D1 group, <sup>**<\/sup><em>P<\/em> &lt; 0.01 <em>vs<\/em> D1 group; <sup>***<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> D1 group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2-3\" class=\"sec\">\n<h3 id=\"s2-3title\">2.3. \u6c22\u6c14\u6c34\u8c03\u8282Nrf2\u4fc3\u8fdbPQ\u67d3\u6bd2\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u589e\u6b96\u4f5c\u7528<\/h3>\n<p class=\"p p-first\">\u901a\u8fc7MTT\u8bd5\u5242\u76d2\uff0c\u68c0\u6d4b\u4e0d\u540c\u5904\u7406\u7684\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u589e\u6b96\u60c5\u51b5\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure3\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure3\" rid-ob=\"ob-Figure3\" co-legend-rid=\"lgnd_Figure3\" rel=\"noopener\"><span>\u56fe 3<\/span><\/a>\uff09\uff0c\u8bbe\u7f6e\u91cd\u590d\u6837\u677f\uff0c\u52a0\u6c22\u6c14\u6c34\u540e\uff0c\u6bcf4 h\u68c0\u6d4b\u4e00\u6b21\uff0c24 h\u52a8\u6001\u5206\u6790\u7ed3\u679c\u663e\u793a\uff0c\u7a7a\u767d\u5bf9\u7167\u7ec4\u7ec6\u80de\u589e\u957f\u8d8b\u52bf\u826f\u597d\uff0cPQ\u67d3\u6bd2\u7ec4\u7ec6\u80de\u589e\u6b96\u660e\u663e\u53d7\u9650\uff1b\u5728PQ\u67d3\u6bd224 h\u540e\u7ed9\u4e0e\u6c22\u6c14\u6c34\u6cbb\u7597\uff0c24 h\u76d1\u6d4b\u53d1\u73b0\u7ec6\u80de\u589e\u957f\u8d8b\u52bf\u660e\u663e\u4e0a\u5347\uff1b\u5f53siRNA\u6291\u5236Nrf2\u57fa\u56e0\u540e\uff0c\u7ed9\u4e0e\u6c22\u6c14\u6c34\u6cbb\u7597PQ 24 h\u540e\u7684HFL-1\u7ec6\u80de\uff0c\u7ec6\u80de\u589e\u957f\u518d\u6b21\u660e\u663e\u53d7\u5230\u6291\u5236\uff0c\u4e14\u4ecb\u4e8ePQ\u67d3\u6bd2\u7ec4\u548c\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u4e4b\u95f4\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure3\" co-legend-rid=\"lgnd_Figure3\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure3\/\" target=\"figure\" rid-figpopup=\"Figure3\" rid-ob=\"ob-Figure3\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234317127776\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-3.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-3.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-3.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234317127776\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure3\/\" target=\"figure\" rid-figpopup=\"Figure3\" rid-ob=\"ob-Figure3\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure3\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure3\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure3\/\" target=\"figure\" rid-figpopup=\"Figure3\" rid-ob=\"ob-Figure3\" rel=\"noopener\">3<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4\u5bf9\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL-1\uff09\u589e\u6b96\u7684\u5f71\u54cd<\/p>\n<p>Effects of different treatments on proliferation of the lung fibroblasts.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2-4\" class=\"sec\">\n<h3 id=\"s2-4title\">2.4. \u6c22\u6c14\u6c34\u8c03\u8282Nrf2\u6291\u5236PQ\u67d3\u6bd2\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u8f6c\u5316\u4f5c\u7528<\/h3>\n<p class=\"p p-first\">Western blot\u68c0\u6d4b\u5404\u7ec4\u03b1-SMA\u7ed3\u679c\u663e\u793a\uff0c\u4e0e\u7a7a\u767d\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0cPQ\u67d3\u6bd2\u7ec4\u808c\u6210\u7ea4\u7ef4\u7ec6\u80de\u6807\u5fd7\u7269\u03b1-SMA\u86cb\u767d\u8868\u8fbe\u91cf\u663e\u8457\u5347\u9ad8\uff0c\u7ed9\u4e88\u6c22\u6c14\u6c34\u6cbb\u7597\u540e\u5176\u8868\u8fbe\u91cf\u663e\u8457\u964d\u4f4e\uff0c\u5728siRNA\u6c89\u9ed8Nrf2\u57fa\u56e0\u540e\uff0c\u5176\u8868\u8fbe\u91cf\u53c8\u8fdb\u4e00\u6b65\u4e0a\u5347\uff0c\u8f83PQ\u67d3\u6bd2\u7ec4\u76f8\u5dee\u4e0d\u660e\u663e\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure4\" rid-ob=\"ob-Figure4\" co-legend-rid=\"lgnd_Figure4\" rel=\"noopener\"><span>\u56fe 4A<\/span><\/a>\uff09\u3002Rt-PCR\u7ed3\u679c\u4e0eWestern blot\u7ed3\u679c\u4e00\u81f4\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure4\" rid-ob=\"ob-Figure4\" co-legend-rid=\"lgnd_Figure4\" rel=\"noopener\"><span>\u56fe 4B<\/span><\/a>\uff09\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure4\" co-legend-rid=\"lgnd_Figure4\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/\" target=\"figure\" rid-figpopup=\"Figure4\" rid-ob=\"ob-Figure4\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234321780608\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-4.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-4.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-4.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234321780608\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/\" target=\"figure\" rid-figpopup=\"Figure4\" rid-ob=\"ob-Figure4\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure4\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure4\/\" target=\"figure\" rid-figpopup=\"Figure4\" rid-ob=\"ob-Figure4\" rel=\"noopener\">4<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4\u4e2d\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u8f6c\u5316\u4f5c\u7528<\/p>\n<p>Pulmonary fibroblast transformation in different treatment groups. D1: Control group; D2: PQ poisoning group; D3: PQ+hydrogen water treatment group; D4: PQ+hydrogen water+siRNA group). <sup>***<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> D1 group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2-5\" class=\"sec\">\n<h3 id=\"s2-5title\">2.5. \u6c22\u6c14\u6c34\u8c03\u8282Nrf2\u6291\u5236PQ\u67d3\u6bd2\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u80f6\u539f\u5206\u6ccc\u7684\u5f71\u54cd<\/h3>\n<p class=\"p p-first\">Western blot\u68c0\u6d4b\u5404\u7ec4Col-\u2160\u3001Col-\u2162\u7ed3\u679c\u663e\u793a\uff0c\u4e0e\u7a7a\u767d\u5bf9\u7167\u7ec4\u76f8\u6bd4\uff0cPQ\u67d3\u6bd2\u7ec4\u80f6\u539f\u86cb\u767d\u7684\u8868\u8fbe\u91cf\u663e\u8457\u5347\u9ad8\uff0c\u7ed9\u4e88\u6c22\u6c14\u6c34\u6cbb\u7597\u540e\u5176\u8868\u8fbe\u91cf\u663e\u8457\u964d\u4f4e\uff0c\u5728siRNA\u6c89\u9ed8Nrf2\u57fa\u56e0\u540e\uff0c\u5176\u8868\u8fbe\u91cf\u53c8\u8fdb\u4e00\u6b65\u4e0a\u5347\uff0c\u8f83PQ\u67d3\u6bd2\u7ec4\u76f8\u5dee\u4e0d\u660e\u663e\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" co-legend-rid=\"lgnd_Figure5\" rel=\"noopener\"><span>\u56fe 5A<\/span><\/a>\uff09\u3002Rt-PCR\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" co-legend-rid=\"lgnd_Figure5\" rel=\"noopener\"><span>\u56fe 5B<\/span><\/a>\uff09\uff0c\u514d\u75ab\u8367\u5149\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" co-legend-rid=\"lgnd_Figure5\" rel=\"noopener\"><span>\u56fe 5C<\/span><\/a>\uff09\u7ed3\u679c\u4e0ewestern blot\u7ed3\u679c\u4e00\u81f4\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure5\" co-legend-rid=\"lgnd_Figure5\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234315885120\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-5.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-5.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-5.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234315885120\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure5\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure5\/\" target=\"figure\" rid-figpopup=\"Figure5\" rid-ob=\"ob-Figure5\" rel=\"noopener\">5<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u80f6\u539f\u7684\u5206\u6ccc<\/p>\n<p>Collagen secretion of lung fibroblasts in different treatment groups (\u00d740). D1: Control group; D2: PQ poisoning group; D3: PQ+hydrogen water treatment group; D4: PQ+hydrogen water+siRNA group. <sup>**<\/sup><em>P<\/em> &lt; 0.01 <em>vs<\/em> D1 group; <sup>***<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> D1 group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s2-6\" class=\"sec sec-last\">\n<h3 id=\"s2-6title\">2.6. \u4e0d\u540c\u5904\u7406\u7ec4\u7ec6\u80de\u6297\u6c27\u5316\u7269SOD\u3001CAT\u3001GSH\u7684\u542b\u91cf<\/h3>\n<p class=\"p p-first\">ELISA\u68c0\u6d4b\u6297\u6c27\u5316\u7269SOD\u3001CAT\u3001GSH\u7684\u542b\u91cf\u7ed3\u679c\u663e\u793a\uff0c\u7a7a\u767d\u5bf9\u7167\u7ec4SOD\u3001CAT\u3001GSH\u86cb\u767d\u8868\u8fbe\u91cf\u6700\u9ad8\uff0cPQ\u67d3\u6bd2\u7ec4\u603b\u4f53\u6700\u4f4e\uff0c\u800cPQ+\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u8868\u8fbe\u91cf\u589e\u52a0\uff0c\u4ecb\u4e8e\u7a7a\u767d\u5bf9\u7167\u7ec4\u4e0e\u67d3\u6bd2\u7ec4\u4e4b\u95f4\uff0cPQ+\u6c22\u6c14\u6c34+siRNA\u7efc\u5408\u5e72\u9884\u7ec4\u8868\u8fbe\u91cf\u8f83PQ\u67d3\u6bd2\u7ec4\u76f8\u5dee\u4e0d\u660e\u663e\uff08<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure6\/\" target=\"figure\" class=\"fig-table-link figpopup\" rid-figpopup=\"Figure6\" rid-ob=\"ob-Figure6\" co-legend-rid=\"lgnd_Figure6\" rel=\"noopener\"><span>\u56fe 6<\/span><\/a>\uff09\u3002<\/p>\n<p><!--fig ft0--><!--fig mode=article f1--><\/p>\n<div class=\"fig iconblock whole_rhythm\" id=\"Figure6\" co-legend-rid=\"lgnd_Figure6\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure6\/\" target=\"figure\" rid-figpopup=\"Figure6\" rid-ob=\"ob-Figure6\" rel=\"noopener\"><!--fig\/graphic|fig\/alternatives\/graphic mode=\"anchored\" m1--><\/p>\n<div class=\"figure\" data-largeobj=\"\" data-largeobj-link-rid=\"largeobj_idm140234315677824\"><img decoding=\"async\" loading=\"lazy\" class=\"fig-image\" alt=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-6.jpg\" title=\"An external file that holds a picture, illustration, etc.\nObject name is nfykdxxb-40-2-233-6.jpg\" src=\"https:\/\/hho-bulgaria.com\/wp-content\/uploads\/2024\/01\/nfykdxxb-40-2-233-6.jpg\"><\/div>\n<p><\/a><\/p>\n<div class=\"largeobj-link align_right\" id=\"largeobj_idm140234315677824\" style=\"display: none;\"><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure6\/\" target=\"figure\" rid-figpopup=\"Figure6\" rid-ob=\"ob-Figure6\" rel=\"noopener\"><\/a><a target=\"object\" rel=\"noopener\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure6\/?report=objectonly\">Open in a separate window<\/a><\/div>\n<div class=\"icnblk_cntnt\" id=\"lgnd_Figure6\">\n<div><a class=\"figpopup\" href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC7086139\/figure\/Figure6\/\" target=\"figure\" rid-figpopup=\"Figure6\" rid-ob=\"ob-Figure6\" rel=\"noopener\">6<\/a><\/div>\n<p><!--caption a7--><\/p>\n<div class=\"caption\">\n<p>\u4e0d\u540c\u5904\u7406\u7ec4\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u6297\u6c27\u5316\u7269\u7684\u8868\u8fbe<\/p>\n<p>Antioxidant expression in the lung fibroblasts in different treatment groups. D1: Control group; D2: PQ poisoning group; D3: PQ + hydrogen water treatment group; D4: PQ + hydrogen water + siRNA group. <sup>**<\/sup><em>P<\/em> &lt; 0.01 <em>vs<\/em> D1 group; <sup>***<\/sup><em>P<\/em> &lt; 0.001 <em>vs<\/em> D1 group.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s3\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"s3title\">3.\u2003\u8ba8\u8bba<\/h2>\n<p class=\"p p-first\">PQ\u4e2d\u6bd2\u5e38\u5bfc\u81f4\u4e25\u91cd\u7684\u80ba\u635f\u4f24\uff0c\u7f13\u89e3\u671f\u80ba\u90e8\u7ec4\u7ec7\u5e38\u53d1\u751f\u7ea4\u7ef4\u5316\u75c5\u53d8\uff0c\u8fd1\u5e74\u6765\uff0c\u591a\u6570\u5b66\u8005\u8ba4\u4e3a\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u662fPQ\u4e2d\u6bd2\u81f4\u80ba\u635f\u4f24\u7684\u91cd\u8981\u56e0\u7d20\u4e4b\u4e00<sup>[<a href=\"#b11\" rid=\"b11\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">11<\/a>&#8211;<a href=\"#b14\" rid=\"b14\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">14<\/a>]<\/sup>\uff0c\u4f46\u5176\u5177\u4f53\u7684\u81f4\u75c5\u673a\u5236\u5c1a\u4e0d\u6e05\u695a\u3002\u5728\u80ba\u7ea4\u7ef4\u5316\u7684\u53d1\u5c55\u8fc7\u7a0b\u4e2d\uff0c\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u5927\u91cf\u5411\u808c\u6210\u7ea4\u7ef4\u7ec6\u80de\u8f6c\u5316\uff0c\u540c\u65f6\u7ec6\u80de\u5916\u57fa\u8d28\u5927\u91cf\u5206\u6ccc<sup>[<a href=\"#b15\" rid=\"b15\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">15<\/a>&#8211;<a href=\"#b17\" rid=\"b17\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">17<\/a>]<\/sup>\u3002\u56e0\u6b64\uff0c\u4ece\u6c27\u5316\u5e94\u6fc0\u53cd\u5e94\u65b9\u5411\u7814\u7a76\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u5728\u80ba\u7ea4\u7ef4\u5316\u4e2d\u5177\u4f53\u7684\u81f4\u75c5\u673a\u5236\uff0c\u5bf9\u4e8e\u5bfb\u627e\u80ba\u7ea4\u7ef4\u5316\u7684\u6709\u6548\u6cbb\u7597\u65b9\u6cd5\u5341\u5206\u91cd\u8981\u3002\u672c\u7814\u7a76\u53d1\u73b0\u6c22\u6c14\u6c34\u53ef\u4ee5\u7f13\u89e3PQ\u6240\u81f4\u7684\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\uff0c\u4e14\u8fd9\u4e00\u4f5c\u7528\u5f88\u53ef\u80fd\u901a\u5bf9Nrf2\u7ec6\u80de\u56e0\u5b50\u7684\u8c03\u8282\u53d1\u6325\u4f5c\u7528\uff0c\u5f97\u51fa\u6c22\u6c14\u6c34\u901a\u8fc7Nrf2\u56e0\u5b50\u51cf\u8f7b\u767e\u8349\u67af\u8bf1\u5bfc\u7684\u80ba\u7ea4\u7ef4\u5316\u7684\u7ed3\u8bba\uff0c\u4e3a\u80ba\u7ea4\u7ef4\u5316\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\u3002<\/p>\n<p>\u6c22\u6c14\u6c34\u5bf9\u7ec6\u80de\u6c27\u5316\u5e94\u6fc0\u635f\u4f24\u5177\u6709\u4fdd\u62a4\u4f5c\u7528\u5df2\u88ab\u591a\u7bc7\u7814\u7a76\u62a5\u5bfc\uff0c\u4f46\u6c22\u6c14\u6c34\u5177\u4f53\u901a\u8fc7\u4f55\u79cd\u673a\u5236\u53d1\u6325\u5176\u6297\u6c27\u5316\u635f\u4f24\u7684\u4f5c\u7528\uff0c\u76ee\u524d\u5c1a\u65e0\u8be6\u7ec6\u62a5\u9053<sup>[<a href=\"#b18\" rid=\"b18\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">18<\/a>]<\/sup>\u3002\u672c\u7814\u7a76\u901a\u8fc7\u4f7f\u7528\u6c22\u6c14\u6c34\u5e72\u9884\u767e\u8349\u67af\u67d3\u6bd2\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff0c\u89c2\u5bdf\u5176\u589e\u6b96\u3001\u8f6c\u5316\u3001\u80f6\u539f\u5206\u6ccc\u53ca\u6297\u6c27\u5316\u7269\u8d28\u7684\u53d8\u5316\u3002\u7ed3\u679c\u53d1\u73b0\uff0c\u6c22\u6c14\u6c34\u5e72\u9884\u540e\uff0c\u67d3\u6bd2\u7ec6\u80de\u7684\u8f6c\u5316\u53ca\u80f6\u539f\u5206\u6ccc\u51cf\u5c11\uff0c\u800c\u7ec6\u80de\u6297\u6c27\u5316\u7269\u8d28\u8868\u8fbe\u91cf\u589e\u591a\uff0c\u4e14\u7f13\u89e3\u4e86\u67d3\u6bd2\u7ec6\u80de\u751f\u957f\u4e0b\u964d\u7684\u8d8b\u52bf\u3002\u6c22\u6c14\u6c34\u901a\u8fc7\u589e\u5f3a\u7ec6\u80de\u7684\u6297\u6c27\u5316\u80fd\u529b\uff0c\u51cf\u8f7b\u4e86\u767e\u8349\u67af\u5bf9\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\u7684\u4f5c\u7528\u3002\u6709\u7814\u7a76<sup>[<a href=\"#b19\" rid=\"b19\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">19<\/a>]<\/sup>\u901a\u8fc7\u56db\u6c2f\u5316\u78b3\uff0c\u786b\u4ee3\u4e59\u9170\u80fa\u548c\u80c6\u7ba1\u7ed3\u624e\u6cbb\u7597\u8bf1\u5bfc\u809d\u7ea4\u7ef4\u5316\u5c0f\u9f20\u6a21\u578b\u540e\u7ed9\u4e88\u5bcc\u6c22\u6c34\u6cbb\u7597\uff0c\u540c\u65f6\u63d0\u53d6\u809d\u7ec6\u80de\u809d\u661f\u72b6\u7ec6\u80de\u4e0e\u6c22\u57f9\u517b\uff0c\u7ed3\u679c\u8bc1\u5b9e\u4e86\u5bcc\u6c22\u6c34\u901a\u8fc7\u6e05\u9664\u7f9f\u81ea\u7531\u57fa\u4fdd\u62a4\u809d\u7ec6\u80de\u514d\u53d7\u635f\u4f24\uff0c\u4ece\u800c\u6291\u5236\u809d\u7ea4\u7ef4\u5316\u5f62\u6210\u3002Gao\u7b49<sup>[<a href=\"#b20\" rid=\"b20\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">20<\/a>]<\/sup>\u901a\u8fc7\u5438\u5165\u6c22\u6c14\u5e72\u9884\u535a\u6765\u9709\u7d20\u8bf1\u5bfc\u7684\u5c0f\u9f20\u80ba\u7ea4\u7ef4\u5316\uff0c\u5f97\u51fa\u6c22\u6c14\u901a\u8fc7\u6291\u5236\u8f6c\u5316\u751f\u957f\u56e0\u5b50\uff08TGF-\u03b2\uff09\u53ca\u6c27\u5316\u5e94\u6fc0\u76f8\u5173\u4fe1\u53f7\u901a\u8def\u7684\u8868\u8fbe\uff0c\u51cf\u5f31\u5c0f\u9f20\u80ba\u7ec4\u7ec7\u7ea4\u7ef4\u5316\u3002\u6b64\u5916, \u76f8\u5173\u7814\u7a76\u8fd8\u6307\u51fa\u9ad8\u6d53\u5ea6\u5438\u6c27\uff0c\u5185\u6bd2\u7d20\u8bf1\u5bfc\u7b49\u9020\u6210\u7684\u80ba\u635f\u4f24\uff0c\u53ef\u4ee5\u901a\u8fc7\u5438\u5165\u6c22\u6c14\u5f97\u4ee5\u6539\u5584<sup>[<a href=\"#b21\" rid=\"b21\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">21<\/a>&#8211;<a href=\"#b23\" rid=\"b23\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">23<\/a>]<\/sup>\u3002\u8fd9\u63d0\u793a\uff0c\u6c22\u6c14\u6c34\u53ef\u4ee5\u901a\u8fc7\u8c03\u8282\u6c27\u5316\u5e94\u6fc0\u7f13\u89e3\u5404\u79cd\u56e0\u7d20\u6240\u81f4\u7684\u80ba\u635f\u4f24\uff0c\u53ef\u80fd\u662f\u6f5c\u5728\u7684\u5e72\u9884\u836f\u7269\uff0c\u4f46\u5176\u5177\u4f53\u7684\u4f5c\u7528\u9776\u70b9\u8fd8\u6709\u5f85\u8fdb\u4e00\u6b65\u7814\u7a76\u3002<\/p>\n<p>Nrf2\u4f5c\u4e3a\u6297\u6c27\u5316\u53cd\u5e94\u5143\u4ef6\u8c03\u63a7\u7684\u4e3b\u8981\u56e0\u5b50\uff0c\u5176\u6297\u6c27\u5316\u548c\u7ec6\u80de\u4fdd\u62a4\u57fa\u56e0\u5728\u80ba\u7ec4\u7ec7\u5bf9\u6297\u6c27\u5316\u6027\u75be\u75c5\u4e2d\u5177\u6709\u91cd\u8981\u7684\u4fdd\u62a4\u4f5c\u7528<sup>[<a href=\"#b24\" rid=\"b24\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">24<\/a>]<\/sup>\u3002\u76f8\u5173\u6587\u732e\u6307\u51fa\uff0cNrf2\u5728\u7c7b\u6bd2\u7d20\uff08LPS\uff09\u6240\u81f4\u7684\u6025\u6027\u80ba\u635f\u4f24\u4e2d\u5177\u6709\u4fdd\u62a4\u4f5c\u7528<sup>[<a href=\"#b25\" rid=\"b25\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">25<\/a>]<\/sup>\uff0c\u540c\u65f6\uff0cH2\u88ab\u62a5\u9053\u901a\u8fc7Nrf2\/HMGB1\u9014\u5f84\u6291\u5236\u8113\u6bd2\u75c7\u5c0f\u9f20\u80ba\u635f\u4f24\uff0c\u6307\u51faNrf2\u5728H2\u6cbb\u7597\u611f\u67d3\u6027\u80ba\u635f\u4f24\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528<sup>[<a href=\"#b26\" rid=\"b26\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">26<\/a>]<\/sup>\u3002\u6709\u7814\u7a76\u53d1\u73b0\uff0c\u4eceNrf2\u7f3a\u5931\u5c0f\u9f20\u4f53\u5185\u63d0\u53d6\u7684\u6210\u7ea4\u7ef4\u7ec6\u80de\u4e0e\u91ce\u751f\u578b\u5c0f\u9f20\u7684\u7ec6\u80de\u76f8\u6bd4\uff0c\u5176\u6297\u6c27\u5316\u9176\u57fa\u56e0\u6c34\u5e73\u4e0b\u964d\u3002\u7ecfPQ\u5904\u7406\u4e24\u7ec4\u7ec6\u80de\u540e\uff0cNrf2\u57fa\u56e0\u7f3a\u5931\u7ec4\u7ec6\u80de\u7684\u6d3b\u6027\u6c27\uff08ROS\uff09\u6c34\u5e73\u660e\u663e\u5347\u9ad8\uff0c\u7ec6\u80de\u5b58\u6d3b\u7387\u964d\u4f4e\uff0c\u800c\u91ce\u751f\u5c0f\u9f20\u7ec4\u7ec6\u80deNrf2\u8868\u8fbe\u589e\u52a0\uff0c\u540c\u65f6\u6297\u6c27\u5316\u7269\u8d28GSH\u6d53\u5ea6\u589e\u9ad8<sup>[<a href=\"#b27\" rid=\"b27\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">27<\/a>&#8211;<a href=\"#b28\" rid=\"b28\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">28<\/a>]<\/sup>\u3002Nrf2\u7684\u5728\u80ba\u7ea4\u7ef4\u5316\u7ec4\u7ec7\u4e2d\u7684\u4e0a\u8c03\uff0c\u5728\u80ba\u7ea4\u7ef4\u5316\u7684\u53d1\u751f\u673a\u5236\u4e2d\u5bf9\u4e8e\u8c03\u63a7\u673a\u4f53\u6c27\u5316\u8fd8\u539f\u72b6\u6001\u53ef\u80fd\u6709\u7740\u81f3\u5173\u91cd\u8981\u7684\u4f5c\u7528\u88ab\u591a\u7bc7\u6587\u732e\u62a5\u9053<sup>[<a href=\"#b29\" rid=\"b29\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">29<\/a>&#8211;<a href=\"#b30\" rid=\"b30\" class=\" bibr popnode\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">30<\/a>]<\/sup>\u3002\u672c\u7814\u7a76\u5047\u8bbe\u6c22\u6c14\u6c34\u53ef\u80fd\u901a\u8fc7\u8c03\u63a7\u6c27\u5316\u5e94\u6fc0\u901a\u8def\u7684\u67d0\u4e2a\u5173\u952e\u4fe1\u53f7\u5206\u5b50\u53d1\u6325\u51cf\u8f7b\u767e\u8349\u67af\u5bf9\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\u7684\u4f5c\u7528\u3002\u901a\u8fc7\u6291\u5236\u6297\u6c27\u5316\u635f\u4f24\u91cd\u8981\u8c03\u8282\u56e0\u5b50Nrf2\u7684\u8868\u8fbe\uff0c\u89c2\u5bdf\u6c22\u6c14\u6c34\u5bf9PQ\u67d3\u6bd2\u7ec4\u7ec6\u80de\u635f\u4f24\u7684\u7597\u6548\uff0c\u53d1\u73b0\u7ec6\u80de\u589e\u957f\u8f83\u5355\u72ec\u7684PQ\u67d3\u6bd2\u7ec4\u518d\u6b21\u660e\u663e\u53d7\u5230\u6291\u5236\uff0c\u7ec6\u80de\u80f6\u539f\u5927\u91cf\u5206\u6ccc\uff0c\u4e14\u6297\u6c27\u5316\u7269\u8d28\u8868\u8fbe\u91cf\u4e5f\u51cf\u5c11\uff0c\u6c22\u6c14\u6c34\u7f13\u89e3\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\u7684\u7597\u6548\u663e\u8457\u964d\u4f4e\u3002\u8fd9\u4e00\u7814\u7a76\u7ed3\u679c\u63d0\u793a\uff0c\u6c22\u6c14\u6c34\u5177\u6709\u51cf\u8f7b\u767e\u8349\u67af\u5bf9\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\u7684\u4f5c\u7528\uff0c\u8fd9\u4e00\u4f5c\u7528\u5f88\u53ef\u80fd\u901a\u8fc7\u5bf9Nrf2\u7ec6\u80de\u56e0\u5b50\u7684\u8c03\u8282\u53d1\u6325\u4f5c\u7528\u3002\u8fdb\u4e00\u6b65\u68c0\u6d4b\u6c22\u6c14\u6c34\u6cbb\u7597\u7ec4\u7ec6\u80deNrf2\u57fa\u56e0\u7684\u8868\u8fbe\u53d1\u73b0\uff0cNrf2\u57fa\u56e0\u8868\u8fbe\u663e\u8457\u589e\u9ad8\uff0c\u8fd9\u53ef\u80fd\u662f\u7531\u4e8e\u6c22\u6c14\u6c34\u5728\u53d1\u6325\u6297\u6c27\u5316\u635f\u4f24\u4f5c\u7528\u7684\u8fc7\u7a0b\u4e2d\uff0c\u8bf1\u5bfcNrf2\u7ec6\u80de\u56e0\u5b50\u7684\u5206\u6ccc\uff0c\u5e76\u4f9d\u8d56Nrf2\u8bf1\u5bfc\u6297\u6c27\u5316\u7269\u8d28\u7684\u5206\u6ccc\uff0c\u4ece\u800c\u53d1\u6325\u6297\u6c27\u5316\u5e94\u6fc0\u4fdd\u62a4\u7ec6\u80de\u7684\u4f5c\u7528\u3002<\/p>\n<p class=\"p p-last\">\u7efc\u4e0a\u6240\u8ff0\uff0c\u672c\u7814\u7a76\u53d1\u73b0\u6c22\u6c14\u6c34\u80fd\u7f13\u89e3PQ\u6240\u81f4\u7684\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u635f\u4f24\uff0c\u540c\u65f6\u80fd\u591f\u8bf1\u5bfc\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80deNrf2\u7684\u8868\u8fbe\u5347\u9ad8\uff0c\u5f53\u7ec6\u80de\u4e2d\u7684Nrf2\u88ab\u6291\u5236\u540e, \u5176\u901a\u8fc7\u589e\u5f3a\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7ec6\u80de\u6297\u6c27\u5316\u80fd\u529b\u4ece\u800c\u51cf\u8f7b\u80ba\u7ec6\u80de\u635f\u4f24\u7684\u7597\u6548\u5927\u5927\u964d\u4f4e\u3002\u672c\u7814\u7a76\u7ed3\u679c\u4ece\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u89d2\u5ea6\uff0c\u63a2\u8ba8\u4e86\u6c22\u6c14\u6c34\u6cbb\u7597PQ\u6240\u81f4\u80ba\u635f\u4f24\u7684\u4f5c\u7528\u673a\u5236\uff0c\u5f97\u51fa\u6c22\u6c14\u6c34\u901a\u8fc7\u4f9d\u8d56Nrf2\u56e0\u5b50\u51cf\u8f7b\u767e\u8349\u67af\u8bf1\u5bfc\u7684\u80ba\u7ea4\u7ef4\u5316\u7684\u7ed3\u8bba\uff0c\u4e3a\u80ba\u7ea4\u7ef4\u5316\u6cbb\u7597\u63d0\u4f9b\u4e86\u65b0\u7684\u601d\u8def\u3002<\/p>\n<\/div>\n<div id=\"d35e54\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"d35e54title\">Biography<\/h2>\n<div class=\"bio\" id=\"d35e54\">\n<div class=\"left\">\u2022&nbsp;<\/div>\n<p class=\"p p-first-last\">\u674e\u5a77\uff0c\u5728\u8bfb\u7855\u58eb\u7814\u7a76\u751f\uff0cE-mail: <a href=\"mailto:dev@null\" data-email=\"moc.qq@2440228481\" class=\"oemail\">moc.qq@2440228481<\/a><\/p>\n<\/div><\/div>\n<div id=\"funding-group-a.h.b.s\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"funding-group-a.h.b.stitle\">Funding Statement<\/h2>\n<p>\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\uff0881560015\uff0c81860018\uff09\uff1b\u4e91\u5357\u7701\u79d1\u6280\u5385-\u6606\u660e\u533b\u79d1\u5927\u5b66\u5e94\u7528\u57fa\u7840\u7814\u7a76\u8054\u5408\u4e13\u9879\u91cd\u70b9\u9879\u76ee\u3001\u9762\u4e0a\u9879\u76ee\uff082017FE468-005\uff0c2017FE468-210\uff0c2014FB046\uff09\uff1b\u4e91\u5357\u7701\u6559\u80b2\u5385\u7814\u7a76\u751f\u9879\u76ee\uff08K13219126\uff09\uff1b\u6606\u660e\u533b\u79d1\u5927\u5b66\u7814\u7a76\u751f\u521b\u65b0\u57fa\u91d1\uff082019S147\uff09<\/p>\n<p>Supported by National Natural Science Foundation of China (81560015, 81860018)<\/p>\n<\/div>\n<div id=\"ref-list-a.j.b\" class=\"tsec sec\">\n<div class=\"goto jig-ncbiinpagenav-goto-container\"><a class=\"tgt_dark page-toc-label jig-ncbiinpagenav-goto-heading\" href=\"#\" title=\"Go to other sections in this page\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Go to:<\/a><\/div>\n<h2 class=\"head no_bottom_margin ui-helper-clearfix\" id=\"ref-list-a.j.btitle\">References<\/h2>\n<div class=\"ref-list-sec sec\" id=\"reference-list\">\n<div class=\"ref-cit-blk half_rhythm\" id=\"b1\">1. <span class=\"element-citation\">Maher TM. 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Curr Opin Pulm Med, 2013, 19(5): 460-5.]<\/span> [<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23912190\" ref=\"reftype=pubmed&amp;article-id=7086139&amp;issue-id=354673&amp;journal-id=3701&amp;FROM=Article%7CCitationRef&amp;TO=Entrez%7CPubMed%7CRecord\">PubMed<\/a>] [<a href=\"\/\/doi.org\/10.1097%2FMCP.0b013e328363f4b7\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=7086139&amp;issue-id=354673&amp;journal-id=3701&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CCrosslink%7CDOI\">CrossRef<\/a>] <span class=\"nowrap\">[<a href=\"https:\/\/scholar.google.com\/scholar_lookup?journal=Curr+Opin+Pulm+Med&amp;title=Beyond+the+diagnosis+of+idiopathic+pulmonary+fibrosis;+the+growing+role+of+systems+biology+and+stratified+medicine&amp;author=TM+Maher&amp;volume=19&amp;issue=5&amp;publication_year=2013&amp;pages=460-5&amp;pmid=23912190&amp;doi=10.1097\/MCP.0b013e328363f4b7&amp;\" target=\"_blank\" rel=\"noopener noreferrer\" ref=\"reftype=other&amp;article-id=7086139&amp;issue-id=354673&amp;journal-id=3701&amp;FROM=Article%7CCitationRef&amp;TO=Content%20Provider%7CLink%7CGoogle%20Scholar\" role=\"button\" aria-expanded=\"false\" aria-haspopup=\"true\">Google Scholar<\/a>]<\/span><\/span><\/div>\n<div class=\"ref-cit-blk half_rhythm\" id=\"b2\">2. <span class=\"element-citation\">Meyer KC. 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Scholar<\/a>]<\/span><\/span><\/div>\n<\/div>\n<\/div>\n<div style=\"display: none; width: 202px; top: -100px; left: -100px;\" aria-live=\"assertive\" aria-hidden=\"true\" class=\"ui-helper-reset ui-ncbipopper-wrapper ui-ncbilinksmenu\">\n<ul id=\"ui-ncbiinpagenav-2\">\n<li><a href=\"#abstract-a.h.b.otitle\">Abstract<\/a><\/li>\n<li><a href=\"#s1title\">1.\u2003\u6750\u6599\u548c\u65b9\u6cd5<\/a><\/li>\n<li><a href=\"#s2title\">2.\u2003\u7ed3\u679c<\/a><\/li>\n<li><a href=\"#s3title\">3.\u2003\u8ba8\u8bba<\/a><\/li>\n<li><a href=\"#d35e54title\">Biography<\/a><\/li>\n<li><a href=\"#funding-group-a.h.b.stitle\">Funding Statement<\/a><\/li>\n<li><a href=\"#ref-list-a.j.btitle\">References<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by enhancing Nrf2 expression<\/p>\n","protected":false},"author":1,"featured_media":17899,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[130],"tags":[],"disease":[875],"body-organ":[1027],"applications":[688],"test_subjects":[1520],"report-topic":[1374],"class_list":["post-26920","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hydrogen-health","disease-environmental-pollution-2","body-organ-lung-2","applications-culture-media-2","test_subjects-cell-culture-2","report-topic-pesticide-toxicity-paraquat-2"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Hydrogen water enhances Nrf2 expression to alleviate lung fibroblast injury<\/title>\n<meta name=\"description\" content=\"Hydrogen water alleviates paraquat-induced lung fibroblast injury in vitro by 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