H2 Water Prevents Oxidative Stress ApoptosisScientific Research

original title: Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water

Authors:

Taichi Kashiwagi, T Hamasaki, S Kabayama, M Takaki, K Teruya, Y Katakura, K Otsubo, S Morisawa, Sanetaka Shirahata

DOI: 10.1007/1-4020-3103-3_50

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Abstract:

We have proposed an active hydrogen reduced water theory that active hydrogen produced by electrolysis of water is stabilized in the form of hydrogenated metal nanocolloids in electrolyzed reduced water (ERW) and scavenges intracellular reactive oxygen species (ROS). Because various brain diseases are caused by oxygen stress, we examined the effect of ERW on oxidative stress-induced apoptois of neuronal cells. ERW suppressed the H2O2-induced cell death of mouse neuroblastoma N1E115 cells, rat pheochromocytoma PC12 cells and mouse neuronal stem SFME cells. ERW lowered the intracellular ROS level of N1E115 cells, suppressing the H2O2-induced decrease of mitochondrial membrane potential and intracellular ATP level, which are markers of apoptosis. These results suggested the effectiveness of ERW for prevention of various brain diseases caused by oxidative stress.

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