Hydrogen Helps Breast CancerScientific Research

H2 Water Slows Tumor Growth and Inhibits Breast Cancer Cells

According to a medical study conducted at multiple universities in Rome, Italy, breast cancer responds well to hydrogen therapy, inducing apoptosis, which is the programmed death of cancer cells.

The study aimed to provide new insights into the effects of hydrogen therapy on breast cancer cell lines, in both – humans and mice.

Laboratory experiments were conducted using hydrogen-saturated water to assess hydrogen’s impact on the growth of human breast cancer cells. Researchers tested three distinct cell lines that represent key models for studying breast cancer development and progression.

These specific cell lines were chosen because they represent different types of breast cancer and have varying characteristics.

The study found that hydrogen treatment inhibited cell survival and induced apoptosis, effectively killing breast cancer cells.

Hydrogen treatment also led to a decrease in the expression of a protein that is often over expressed in breast cancer.

Furthermore, hydrogen use disrupted the activation of a signaling pathway involved in cancer cell growth and survival.

Overall, this study provides new evidence for the potential anticancer effects of hydrogen on breast cancer cells and suggests that its mechanism of action involves antioxidant properties and modulation of the expression of a receptor protein that plays a critical role in breast cancer.

The study also investigated the effect of hydrogen on proteins found on the surface of breast cancer cells, which play a crucial role in the growth and survival of these cells.

In vivo experiments were also conducted using transgenic mice that develop breast tumors.

The study found that hydrogen therapy significantly extended tumor-free survival in these mice. This means that hydrogen use increased lifespan without tumor development. Additionally, hydrogen therapy reduced the number of tumors, demonstrating its potential to inhibit tumor growth.

In this study, Italian researchers showed that hydrogen therapy inhibits cell survival, induces cancer cell apoptosis, decreases protein expression, and disrupts protein kinase activation in breast cancer cells. Additionally, in vivo study results showed that hydrogen induced a significant extension of tumor-free survival and helped reduce multiple tumors.

These findings provide promising evidence for the potential beneficial role of hydrogen therapy in inhibiting breast cancer cell growth.

The Original Article:

original title: Electrochemically Reduced Water Delays Mammary Tumors Growth in Mice and Inhibits Breast Cancer Cells Survival In Vitro


Giovanni Vanni Frajese, Monica Benvenuto, Rosanna Mattera, Saverio Giampaoli, Elena Ambrosin, Roberta Bernardini, Maria Gabriella Giganti, Loredana Albonici, Ivan Dus, Vittorio Manzari, Andrea Modesti, Maurizio Mattei, Roberto Bei

DOI: 10.1155/2018/4753507



Electrochemical reduced water (ERW) has been proposed to have beneficial effects on human health due to its rich content of H 2 and the presence of platinum nanoparticles with antioxidant effects. Many studies have demonstrated that ERW scavenging properties are able to reduce the damage caused by oxidative stress in different experimental models. Although few in vivo studies have been reported, it has been demonstrated that ERW may display anticancer effects by induction of tumor cells apoptosis and reduction of both angiogenesis and inflammation. In this study, we show that ERW treatment of MCF-7, MDA-MB-453, and mouse (TUBO) breast cancer cells inhibited cell survival in a time-dependent fashion. ERW decreased ErbB2/ neu expression and impaired pERK1/ERK2 and AKT phosphorylation in breast cancer cells. In addition, ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors. Our in vivo results showed that ERW treatment of transgenic BALB- neu T mice delayed the development of mammary tumors compared to the control. In addition, ERW induced a significant prolongation of tumor-free survival and a reduction in tumor multiplicity. Overall, these results suggest a potential beneficial role of ERW in inhibiting cancer cells growth.

Original Publication